Study On The Structure And Biological Activity Of Four Lanthanide Complexes Bonded By Pyridine Or Pyridine Carboxylic Acid Ligands

The discovery of the anti-tumor effect of cisplatin led to the birth of metal-based chemotherapeutics.Platinum drugs such as cisplatin,oxaliplatin and carboplatin have been verified to be used alone or in combination with other drugs to treat various malignant tumors.However,due to drug resistance and toxicity,the application of cisplatin is limited.Therefore,the anti-cancer potential of some non-platinum metal drugs,such as transition complexes and rare earth complexes,has slowly been studied.As an electron donor,N-containing ligands can effectively coordinate with a variety of metals.The complexes that are stable under physiological conditions can be obtained through standard synthesis methods,thus providing new ideas for many fields such as biology and inorganic chemistry.It has shown great potential in the development of inorganic medicinal chemistry and metal-based drugs.It is expected that more and more NHC ligands will be considered in the design of metal drugs,which may become candidates for clinical trials in the future.In short,nitrogen heterocyclic ligands provide new ideas for drug development.Therefore,this topic has synthesized four new structural complexes by hydrothermal method,and conducted comprehensive discussion on the anti-tumor effect.The specific research contents are as follows:1.We chose 1,1’-(1,4-phenylene-bis(methylene))-bis(pyridine-3-carboxylic acid)1,4-bis(2-(4-pyridinio)ethenyl)benzene as ligand,and Pr,Er,Ho,Yb complexes were synthesized.The crystal related information was confirmed by X-ray single crystal diffractometer;2.The elemental composition of the complex was measured by elemental analyzer,the structure of the synthesized complex was verified by an infrared spectrometer,and the synthesized crystal was determined to be the target product by an X-ray powder diffractometer;3.Fluorescence spectroscopy and ultraviolet-visible spectroscopy proved that complexes 1-4 can be combined with FS-DNA,with strong or weak binding ability,but both proved that the ability of complex 1 is better than that of complex 2,and the ability of complex 4 is better than that of complex 34.The agarose gel electrophoresis method proved that all four complexes can cleave FS-DNA,but the cutting ability is different.Molecular docking simulation technology shows that the four complexes can interact with the base pair of DNA.5.The ability of the complex to induce He La cell apoptosis was detected by flow cytometry.The results also verified that the apoptotic ability of complexes 1 and 4 was better than that of 2 and 3.The fluorescence inverted microscope observed He La from the morphological changes.