Study On Erythrocyte Membrane Or Virus-like Particles As Carriers For Tumor Photothermal Therapy

The use of drugs for cancer diagnosis and treatment is usually limited by the shortcomings such as the poor solubility in aqueous solution,the short retention time in blood,and the immune response.Modification using polyethylene glycol(PEG)is a tranditional strategy for cancer diagnosis and treatment agents to improve the stability of the agents in vivo.However,the application is limited by complicated protocol,anti-PEG immune response,and potential kidney damage.Therefore,two biomaterials,red blood cell(RBC)membrane bionic vesicles and p H-sensitive virus-like particles(VLP),are prepared in this paper,and their potential for modifying cancer diagnostic and therapeutic agents is explored.Firstly,by preparing erythrocyte membrane vesicle encapsulated photocatalyst titanium dioxide colloid and photothermal agent gold nanorods(Au NRs),bionic nanocapsules Au/Ti O2@RBC are obtained,and the controlled release of Au NRs can be achieved through photocatalytic degradation of the surface cell membrane coating.Near infrared(NIR)laser irradiation produces a photothermal effect,and reactive oxygen species(ROS)produced by photocatalysis achieve photodynamic therapy(PDT).Secondly,to solve the problems of poor solubility,poor stability,difficult absorption,and fast metabolism of curcumin in water,a red blood cell membrane bionic nano drug-loading system(Au-Cur@RBC)is designed.Gold nanoparticles with uniform size and good dispersibility was prepared,which has an average particle size of 13.18 nm.The encapsulation rate of curcumin in gold-curcumin nanoparticles was 86.85%,and the drug loading rate was 20.25%.Through the loading of gold nanoparticles,the stability of curcumin can be significantly improved.After storage of gold-curcumin nanoparticles in an aqueous solution for30 days,curcumin still retains 52%.Meanwhile,the results of dark field microscopy have showed that the red cell membrane coating can improve the ability of the prepared Au-Cur@RBC to refract and diffract the incident light of the dark field microscope,thereby presenting a clearer image.Finally,8 aspartic acids and 8 arginines were introduced into the murine polyoma virus(MPV)structural protein VP1 to construct positively charged VP1 and negatively charged VP1,respectively.The self-assembly of mutant VLP(m VLP)in vitro and its response to the p H of solution are examined.The results show that m VLP can be disassembled at p H 6.5,which has the potential to achieve controlled release of drugs in the tumor microenvironment.