| In this project,macromolecular chitosan was degraded into small molecular water-soluble chitosan by supported phosphotungstic acid catalyst in heterogeneous system from natural biomaterial chitosan.The degradation process of water-soluble chitosan was optimized by response surface analysis.then,small molecule water-soluble chitosan and SA were covalently formed into an amphiphilic chitosan derivative using1-(3-dimethylaminopropyl)-3-ethylcarbodiimide salt(EDC HCl)as catalyst.the amphiphilic chitosan derivative was self-assembled to form CS-SA nanomicelles under certain conditions.finally,the hydrophobic drug florfenicol(FF)was coated in CS-SA nanomicelles to prepare FF/CS-SA nanomicelles.In this paper,the stability of FF / cs-sa nano micelles was tested in high temperature,high humidity,strong light,accelerated test for 6 months and long-term test for 12 months.The pharmacokinetics of FF / cs-sa nano micelles was studied in SD rats by intravenous injection and oral administration.Stability of FF/CS-SA nanomicelles was investigated in this project.The pharmacokinetics of FF/CS-SA nanomicelles was studied in SD rats by tail vein injection and oral administration.The results showed that under high temperature,high humidity,strong light irradiation and accelerated test for 6 months and long-term test for 12 months,all the indexes of FF/CS-SA nano-micelle met the requirements,indicating that the stability of FF/CS-SA nano-micelle was good,and the pharmacokinetic characteristics of FF/CS-SA nano-micelle were in accordance with the one-compartment open-room model.Compared with FF common preparations,the half-life of FF/CS-SA nano-micelle in rats was prolonged,the product under the drug-time curve was AUC prolonged,the residence time was prolonged,and the bioavailability was improved.FF/CS-SA nano-micelle is a promising new preparation.and this laid a certain working foundation for the clinical practical application of FF/CS-SA nanomicelles. |
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