Applications Of Infrared Hybrid Spectroscopy In Spectral Separation Of Multiple Components

Infrared spectroscopy,as a very important analysis and identification method in chemical research,can provide abundant material structure information.One of the characteristics of infrared spectroscopy is that the applicability of the sample is very wide,no matter gas,liquid,solid can be measured by infrared spectroscopy.In addition,infrared spectroscopy is widely used in chemistry,biology,medicine,materials,astronomy,optics,meteorology,physics and other fields due to the characteristics of rapid testing,convenient operation,good repetition rate,and less sample usage.Multi-component system is often encountered during the measurement of infrared spectroscopy.When the infrared absorption peaks of different components overlap,it will have a serious adverse effect on our qualitative and quantitative analysis.For this reason,this paper introduces the concept of hybrid spectrum,which is obtained by linear combination of different concentration spectra of two same samples.Different hybrid spectra can be obtained by changing the hybridization factor(this article uses changing the number of scans to change the hybridization factor),so we can use hybrid spectra to replace sample spectra with any concentration in this concentration range.Based on this unique advantage of hybrid spectrum,we introduce hybrid spectrum to the multi-component spectral separation.When collecting the solution attenuated total reflectance(ATR)infrared spectroscopy,select the double background sample,empty ATR as background 1,solvent ATR as background 2,empty ATR scanning 32 times and then pause,and then continue to scan solvent ATR background.As the number of scans for background 2 increases,the solvent peak gradually becomes weaker until the solvent peak disappears,and the solvent peak in the solution is deducted online.The experimental results show that the hybrid spectrum can successfully subtract the absorption peak of the solvent in the solution,the water peak in DMSO,the water peak in bovine serum albumin(BSA)solution,and the water peak in monosaccharide and disaccharide solutions.The deduction results are obviously good than spectral subtraction technology.The hybrid spectrum can obtain satisfactory results in both the Lambert-Beer law system and the non-Lambert-Beer law system.It solves the defect that the spectral subtraction technology is not applicable to the non-Lambert-Beer law system,and provides a new measurement method for infrared spectroscopy.