Preparation Of Fluorescent Probes With Aggregation-induced Emission And Their Detection Of Phosgene And Hydrogen Sulfide

Fluorescent probes with aggregation-induced emission have many advantages,such as strong emission in the aggregated state upon responding to specific analytes and good photobleaching resistance,hence they are conducive to improving the performance for detection and imaging in aqueous media,and thus their applications in the fields of environment monitoring,chemical sensing and bio-detection have received more and more attention.Herein,two kinds of AIE fluorescent probes（TPE-Phos and TPET-NO₂@BSA）have been designed and prepared for the detection of phosgene and hydrogen sulfide respectively,and the main contents are as follows:（1）A ratiometric AIE-type fluorescent probe（TPE-Phos）for phosgene detection with oxime as the recognition group has been designed and synthesized.The probe molecules TPE-Phos are deposited onto filter paper to prepare the fluorescent test paper for detecting phosgene.Upon contact with phosgene,the test paper’s emission wavelength red-shifts from 470 nm to542 nm.Under the irradiation of 365 nm UV light,the fluorescence color of the test paper changes from blue to yellow.The response of the test paper to phosgene is fast and sensitive.In addition,the response of the test paper to phosgene exhibits relatively good selectivity and anti-interference,and the response won’t be interfered by formaldehyde or nitric oxide.（2）An AIE-type activable nanoprobe TPET-NO₂@BSA has been designed and employed for detecting H₂S.The nanoprobe reacts with H₂S,and correspondingly 2,4-dinitrophenyl ether is cleaved with hydroxyl group being generated,and thereby strong fluorescent signals are given out.Moreover,the nanoprobe TPET-NO₂@BSA has relatively good biocompatibility.The nanoprobe TPET-NO₂@BSA shows low detection limit（85 nM）for H₂S detection.In addition,the nanoprobe can be used to detect and image endogenous H₂S in living cells as well as image H₂S in the subcutaneous tumor in mouse model.