Study On The Interaction Of Fluorinated Graphene With Protein And Cell Membrane

Graphene is a single-layer carbon atom with a honeycomb lattice structure.In recent years,due to its unique kinetics,thermodynamics and excellent biological properties,it has received extensive attention and has carried out corresponding research,which has promoted its development.Applications in aerospace,desalination,biomedicine,etc.With the development of material science,fluorinated graphene（F-GRA）has gradually entered people’s field of vision.It is an emerging member of the graphene family and an important derivative of graphene with a two-dimensional layered structure,wide band gap and high stability,and have attracted much attention due to their unique nanostructure and carbon-fluorine bonds.Due to its excellent properties,such as high strength,large specific surface area,excellent lubricity,hydrophobicity and antiwear properties,and wide band gap,it has become a research hotspot.Studies have shown that fluorinated graphene with different sizes,layers,degrees of fluorination and functional groups exhibits different optical,electrical,thermal and interface properties,so it can be widely used in solid lubricating materials,anti-fouling and anti-fouling.Corrosive materials,lithium fluoride battery cathode materials,superhydrophobic materials and wear-resistant materials,and have potential applications in nanoelectronic devices,optoelectronic devices and other fields.F-GRA has been widely used in the field of biomedicine and exhibits excellent biological functions,especially many biological effects caused by its direct interaction with various biomolecules.Nevertheless,so far,there are not enough theoretical reports describing the interaction between F-GRA and biomolecules and the biological effects at the molecular level.This paper focuses on the frontier of international scientific research on nano-biological effects（especially nano-biological safety）,using theoretical simulation,combined with the knowledge of interface physics and biophysics,to fully characterize the microscopic behavior of the interface between F-GRA materials and biomolecules.In this study,we investigated the adsorption of F-GRA on phospholipid bilayers and the effect on protein structure using molecular dynamics simulations to evaluate the potential effects of F-GRA materials on biological membranes and proteins and their mechanisms.Our results indicate that（1）In the adsorption process to phospholipid bilayers,F-GRA can be partially intercalated into the membrane or adsorbed in parallel on the membrane surface,unlike the intercalation of graphene.Detailed analysis confirmed that electrostatic force mainly mediates the adsorption process.The parallel binding of F-GRA resulted in increased membrane thickness by disrupting the lipid order parameter,indicating that its impact on membrane structural stability cannot be ignored.This perturbation is comparable to that observed in the graphene case,although the membrane integrity in parallel adsorption is not significantly altered.（2）In the process of binding with the protein,unlike graphene,which will destroy the protein structure and denature it,the protein can maintain a good structural integrity after being adsorbed on the surface of F-GRA,indicating that it has better biocompatibility with the protein.These findings suggest that F-GRA has mechanical perturbation of cell membranes,that is,potential toxicity,and good biocompatibility with proteins.These studies provide a prospective scientific basis for enriching the physical nature of the biological effects of F-GRA,provide a theoretical basis for the design of F-GRA-related nanomedicines in the future,and are expected to become nanomaterials with great prospects in future biomedical applications.