Photo-initiation Preparation And Application Of Betaine Type Zwitterionic Monolithic Column

Zwitterionic compounds are small molecules that simultaneously contain equal amounts of opposite charges.At present,zwitterionic functional materials have been widely developed in medicine,engineering,biology,and separation science,especially used in hydrophilic interaction chromatography,due to their anti-pollution,high biocompatibility and strong hydrophilicity.Monolithic column,which be describes as“the fourth generation of chromatographic column”.Compared with the traditional packed column,it has the advantages of simple preparation,good permeability,high mechanical strength,easy modification and high separation efficiency.Combining with zwitterionic stationary phase,the development of new zwitterionic hydrophilic monolithic column has been a research focus.In this paper,based on the above points,two new zwitterionic hydrophilic monolithic columns were successfully prepared and related applications were studied.Chapter one,the zwitterionic materials,hydrophilic interaction chromatography and monolithic column were systematically described.The stationary phases used in hydrophilic interaction chromatography(zwitterionic stationary phase and nonzwitterionic stationary phase)were introduced in detail,and the monolithic column,especially the organic polymer monolithic column,was explained in detail.The significance of developing the rapid preparation of single cross-linking zwitterionic hydrophilic monolithic column was explained.Chapter two,two zwitterionic monomers,4-(3-vinyl-1-imidazolio)-1-propanesulfonate(SBVI)and 1-Carboxy-N-methyl-N-di(2-methacryloyloxy-ethyl)methanaminium inner salt(CBMAX)were synthesized and characterized by HNMR,NMR carbon,mass spectrometry and elemental analysis.The development of novel zwitterionic monomers have enriched the field of zwitterionic stationary phases.Chapter three,SBVI was functional monomer,pentaerythritol triacrylate(PETA)as crosslinking agent,methanol(Me OH)and tetrahydrofuran(THF)were porogens,benzoin dimethyl ether(DMPA)as the photoinitiator,the system was optimization,through polymerization reaction for 5 minutes,preparation for poly(SBVI-co-PETA)monolithic column.The morphology of the monolithic column was characterized by SEM,EDS mapping and pore size distribution,and the physical and chemical parameters such as mechanical strength,permeability,reproducibility,Van Deemter equation and retention mechanism were tested.Finally,the monolithic column was applied to separation,anti-protein adhesion and glycopeptide enrichment.Poly(SBVIco-PETA)monolithic column not only had good performance,but also used the means of photopolymerization,and this preparation method was simpler and faster than the traditional thermal polymerization.Chapter four,poly CBMAX single-crosslinking zwitterionic monolithic column was prepared by using CBMAX as single-crosslinking functional monomer,Me OH and THF as porogens and DMPA as photoinitiator.After system optimization,poly CBMAX monolithic column was prepared by photopolymerization reaction for 5 minutes.Physical and chemical parameters were tested such as the mechanical strength,permeability,Van Deemter equation,and the repeatability of the retention mechanism.Also through a series of experiments on the characterization of morphology.Using poly CBMAX monolithic column has realized the separation of polar compounds.They were also applied to anti-protein adhesion and glycopeptide enrichment.On the one hand,poly CBMAX monolithic column enriched the zwitterionic stationary phases,on the other hand,the preparation system was simplified by single-crosslinking,and the performance was still improved to some extent.Chapter five,the main research content of the whole paper was systematically summarized,the shortcomings of this study were proposed,and on this basis,the future research work was prospected.