Novel Dual-emissive Fluorescent Silicon Nanoparticles For Detection Of Enzyme Activity In Supplements

Silicon nanomaterials have aroused great interest of researchers due to their excellent electronic,optical and mechanical properties.In the past decades,researchers have devoted themselves to developing methods for the synthesis and functionalization of silicon nanomaterials with different nanostructures.Currently available silicon nanomaterials include but are not limited to silicon nanowires,silicon nanorods,and silicon nanoparticles（SiNPs）.Compared with traditional light-emitting quantum dots which contain heavy metals,zero-dimensional fluorescent SiNPs have the properties of simple synthesis,low cytotoxicity,high biocompatibility,high resistance to photobleaching and good photostability.Now,the fluorescent SiNPs are widely used in the constructions of fluorescent probes,biomarker detection,etc.A novel kind of dual-emissive Rhodamine 6G modifed silicon nanoparticles（SiNPs-Rho6G）was synthesized through a one-pot hydrothermal method in the dissertation.This dual-emissive SiNPs-Rho6G with emission wavelength at 415/550nm under 320 nm excitation possessed the advantages of strong fluorescence and photostability.SiNPs-Rho6G was developed to set up a ratiometric fluorescence sensor towardβ-Galactosidase（β-Gal）activity with p-Nitrophenol（PNP）involved.The activity ofβ-Gal was determined in a range of 1-25 U·L^-1 with a limit of detection of 0.72 U·L^-1.The proposed method was further utilized to determine theβ-Gal activity in enzyme formulations with high sensitivity and selectivity.Moreover,this method was proved to have the potential of being an analysis platform for various target molecules in the fields of medicine,food and drug.