Selective Extraction And Separation Of Luteolin By Metal-modified Boronate-affinity Adsorbent

China is a large agricultural country,peanut shell is one of the main agricultural wastes.Except a small amount is recycled,most of it is burned directly.This not only causes environmental pollution,but also leads to the waste of resources to a certain extent.Studies show that peanut shell contains rich luteolin,which is a kind of flavonoids with cis-diol structure and has great medicinal value.However,the substrate of peanut shell is relatively complex and there are many interference components,the response signal of luteolin is easily affected and difficult to be detected directly.Therefore,sample pretreatment is necessary before analysis and detection,and solid phase extraction（SPE）technology stands out among them for its higher enrichment multiple,less solvent consumption and higher recovery rate.Boronate affinity technology and metal affinity technology are two typical extraction methods of cis-diols.In this paper,a SPE technique combining these two kinds of affinity capabilities is proposed to be used for the pretreatment of complex samples,in order to achieve the purpose of simple and rapid separation and analysis.On this basis,two new sorbents for SPE were established:1.Amorphous Si O₂ was used as a matrix,Zr O₂ was coated by solvothermal synthesis method as a metal affinity functional monomer,3-aminobenzenoborate was introduced as a boronate affinity functional monomer,and molecular imprinting was carried out by using the self-polymerization ability of dopamine.The synergistic and amphiphilic molecularly imprinted polymers with high selectivity were prepared.2.The cobalt-based zeolite imidazole framework was used as a matrix and the self-polymerization ability of dopamine was used to form a coating layer.Then,the3-carboxyl phenylboric acid modified zirconium-based metal-organic frameworks were fixed on the suface of materials by a large number of amino and hydroxyl functional groups,and zeolite imidazole framework-based boronic acid-functionalized metal-organic frameworks were obtained for adsorption.A series of characterization and optimization of the above two adsorbents were carried out,and they were successfully applied to the separation and enrichment of luteolin in peanut shell.The experimental results verified the excellent affinity and selectivity of the two methods,and provided a new idea for the analysis and detection of other cis-diol substances.