Research On Droplet Manipulation Surface And Applications Based On Electric Response And Self-Driving

Droplet manipulation is of paramount significance for microfluidics based biochips,especially for bioanalytical chips.Despite great progresses made on droplet manipulation,the existing bioanalytical methods face challenges in terms of capturing minute doses toward hard to obtain samples and analyzing biological samples at low temperatures immediately.To circumvent these difficulties,research combines bionic strategies,external stimulation,and the advantage of femtosecond laser precision manufacturing process(a non-contact and highly integrated control precision surface process,which has become one of the indispensable surface processes in the micro-/nano manufacturing industry because it extremely small heat-affected area and extremely short pulse length.),prepares a droplet manipulation surface that can achieve spontaneous and controllable droplet manipulation for microsampling detection.Moreover,the droplet transportation(delaying icing)and microreagent detection can also be realized in low temperature environment(-5℃),which has very good application prospects.The main contents are as follows:First,the differences of different laser processes,the construction of femtosecond laser process platform,experimental instruments and materials are introduced.Second,a uniform and controllable micro-/nanostructures are prepared on the surface of different materials by femtosecond laser processes.Finally,the influence of femtosecond laser parameters(laser spot,laser power,and pulse number)and the surface structure of the substrate are systematically discussed,and the optimal processing parameters for this study are obtained.Based on the study of the structure of the Solanum khasianum C.B.Clarke and the function of the Nepenthes plant,the surface for droplet passive control was prepared by the dual bionic strategies.In addition,the factors of affecting passive control of the droplet such as laser pulses,microbowl spacing,lubricating oil thickness,and the apex angle of substrate are analyzed,and the best interval of parameters is obtained.The effects of voltage on droplet wettability are analyzed by combining external stimulus and the study of passive control surface parameters.Moreover,the charge distribution of droplets on different structures is studied by the multi-physical field simulation.Furthermore,the potential theoretical mechanism in dual-driven droplet manipulation was studied.Finally,a dual-driven surface for multi-type droplets and different temperature droplets manipulation is prepared according to the optimum interval of the parameters.The applications of droplet manipulation in biomedicine and existing diagnostic methods is summarized.Meanwhile,for existing issues such as difficulty in detection,real-time convenient detection of heavy metal ions in the water environment,a microsampling detection device is prepared which is adapt to different ambient temperatures.