Research And Device Design Of Portable Multi-mode Microscopy Imaging Technology

With the continuous development of microscopic imaging technology,optical microscopy has a series of imaging technologies,including bright field,dark field,polarized light,phase contrast,differential interference,fluorescence and so on.At present,brightfield,darkfield and phase contrast microscopes are still the most widely used imaging methods,but for translucent samples,such as unlabeled cells and thin tissue specimens,brightfield imaging methods are difficult to obtain ideal experimental images.The use of illuminating light exceeding the angle corresponding to the numerical aperture of the optical imaging system can realize dark-field imaging,thereby can produce high-contrast images.In addition,the observation of organelles with subtle phase changes requires differential interference and phase contrast imaging methods.Whether it is dark field or differential interference and phase contrast imaging methods,additional optical components are required.Such microscopes are generally bulky,expensive,and difficult to meet the needs of rural primary medical inspections,and they cannot be used in mobile medicine and field medicine.Therefore,the development of a portable microscope that can achieve multi-mode imaging at the same time will be of great significance to the development of rural primary care and field medical clinical testing.In addition,if there is an integration of automatic focus and scanning imaging,it will be more conducive and can simplify the complexity of the inspection personnel,reduce human deviation.In response to the above needs and problems,this article focuses on the research of imaging technology and the design of portable devices,and quantitatively evaluate the influence of shading ratio on oblique illumination microscopic imaging,combined with the commercial Olympus IX73 microscope.The sliding diaphragm is driven by a stepper motor.Gradually increase the shading ratio of the imaging illumination,collect images at the same time,use the local contrast and Laplacian matrix variance to quantitatively evaluate the imaging quality,and find the best oblique illumination shading ratio,which is similar to the imaging effect of DIC.This research does not require the insertion of complex optical components in imaging without lots of calculations.Therefore,it can be used for real-time monitoring to effectively improve image contrast while reducing costs.It is expected to provide new ideas for biological sample detection and real-time high-contrast cell monitoring.In addition,the Olympus IX73 microscope light source is replaced with a coded LED array for illumination.By calculating the relationship between the illumination angle and the height of the illumination aperture,the LED illumination model can be flexibly adjusted to realize bright field,dark field,oblique illumination,differential phase contrast and quantitative phase imaging.Besides we also have a discussion about how to achieve multi-mode imaging with a single exposure.At the same time,we fabricate a portable multi-mode fully automatic microscope imaging system.The size of the entire device is 266*216*216 cm.The self-made spherical LED array is used for coded illumination,which can realize multi-mode imaging of bright field,oblique illumination,differential phase contrast and dark field at the same time.Through mechanical design,single-chip control and self-editing control software,a miniaturized two-dimensional electronically controlled microscopy platform is realized.The improved Roberts gradient function value is used to judge the imaging quality and obtain the best imaging surface.Combined with motor control,it realizes portable fully automatic focusing and scanning imaging.At the same time,a complete set of Lab VIEW-based portable multi-mode microscopy imaging system control program has been developed,which can realize the illumination modulation of the microscope system,camera parameter adjustment,two-dimensional scanning motion control,automatic focus control,and multi-mode real-time Personalized shooting,preservation and video recording of imaging and experimental images.Finally,various parts of the system are tested,and the accuracy and reliability of the system are verified by samples such as mouse testicular tissue slices and USAF resolution test chart.The device achieves the advantages of low cost,simple operation and portability,and provides real-time diagnostic tools for resource-poor areas and field mobile medical situations.