Inactivation Kinetics And Regrowth Potential Of Antibiotic Resistance And Non-Resistance E.coli With Performic Acid And Peracetic Acid

Water has profound importance in human lives,its limited and depleting resources has made the world emphasize on its reclamation or reuse.Peracetic Acid（PAA）and Performic Acid（PFA）are powerful disinfectants having approximately the same oxidation potential,PAA being used widely as a disinfectant in water disinfection.Antibiotics are extensively used pharmaceuticals and discharged into water streams,posing a potential risk as a micropollutant to human health,by developing antibiotic resistance in bacteria.Therefore,their study has great importance;their interactive actions with the bacteria in the water streams and ultimate decomposition pathways.In this study,the antibiotic resistance（AR）and non-resistance Escherichia coli（E.coli）was treated with PAA（1.5mg/L 14min）and PFA（1.7mg/L 14,16min contact time）separately and combined surrogates and their inactivation kinetics being compared and statistical analysis being held by using paired t-test.For this purpose,AR E.coli and non-resistance E.coli is used due to their simple structure and short generation time.In addition to that the regrowth/reactivation potential（CFU/ml）also being checked and compared post disinfection by using a range of growth parameters namely;Luria Broth（0,30ug/ml）and PBS（0,30ug/ml）.The reactivation potential was checked and compared statistically（two-way ANOVA）,for the joint and individual regrowth of E.coli post disinfection by PAA and PFA.The regrowth potential of PFA and PAA also evaluated and compared suggesting a regrowth elevation in the presence of antibiotics and nutrient abundance medium（LB）whereas,denoting a negligible growth in nutrient deficit medium（PBS）for AR E.coli and their joint growth.Moreover,the spectrophotometric quantification of PFA and PAA is reported which isn’t conducted before,for PFA.Moreover,very limited data is available on PFA disinfection in water.The effect of Fe³⁺,a commonly used coagulant（Fe Cl₃）is also studied,suggesting that it has an ultimate effect on prolongation of contact time during disinfection.The research focusing,on the inactivation kinetics and growth comparisons of AR E.coli and non-resistance E.coli as well as evaluating the regrowth potential of PFA isn’t being reported previously.The aim of this study is to compare the inactivation kinetics and regrowth potential of antibiotic resistance and non-resistance E.coli,the quick quantification of PFA and PAA and providing more insights of the PFA as a disinfectant.