The Identification Of Soybean MiRNA In Response To Aluminum Stress And The Heterologous Expression Of GmPDR12 In Arabidopsis

Recently,the frequent acid rain and the large use of acidic chemical fertilizer make the area of acid soil and the acidification degree increased greatly.In the acid rain,Al dissolves out in the bigger toxicity form,which influences the growth of crops seriously and limits the development of the world agriculture.In order to make crops grow in the acidic soil normally,scientists from different countries conducted a wide range of researches,including the Al toxicity to plants and the tolerance of plants to Al toxicity.miRNA plays crucial role in regulating the expression of target genes.To discover the soybean miRNAs in response to Al stress and dissect their functions will lay theoretical foundation on deeply revealing the molecular mechanism of plants in resistant to Al stress.The present study identified dozens of Al-responsive miRNAs by high throughput sequencing from two soybean genotypes and some of them were validated by RT-qPCR method.Comparative analysis of four libraries’data sets revealed that 9 miRNAs(166k,166u,169r,396i-5p.396e,398,1520q,4397-3p 和5044)were significantly down-regulated in Al-tolerant soybean genotype.Future investigations should focus on their targets verification and their regulatory network in response to Al stress and illuminated their molecular mechanism in plant resistance.Based on previous study in our lab,I cloned a ABC transporter gene,GmPDR12,from Al-resistant soybean genotype-BX10 and constructed its expression vector in my Bachelor’s period.In the present study,the expression vector was transformed into Agrobacterium tumefaciens GV3101.Then the Arabidopsis plant were infected with GV3101,and the positive transgenic plants were screened.Next,we will focus on the detailed molecular mechanism of GmPDR12 in plants’ resistance to Al stress with the transgenic Arabidopsis plant as well as the soybean plants to be transformed.