| Climate change is one of the most serious problems in the contemporary,in the past two centuries,CO2 and other greenhouse gases caused from human activity changed atmospheric composition and surface properties,and then change the gas energy of the system of payments and caused the global climate change.Alternanthera philoxeroides(Amaranthaceae)is native from South American,and is an herbaceous,amphibious weed,because it can invade many habitats and grow rapid,it is difficult to control,and threatened to the biodiversity,ecosystem and social economy.A.hygyophila is the special natural enemy of A.philoxeroides,and the feeding and controlling effect of A.philoxeroides is remarkable.The nutrition substances of fresh leaves of A.philoxeroides grown in different concentrations of CO2 were determined.The results showed that the content of free fatty acids of A.philoxeroides decreased at 550 ppm and increased at 750 ppm.The content of starch increased with the increase of CO2 concentration.The content of soluble sugar decreased under 550 ppm and increased under 750 ppm.The content of protein decreased with the increase of CO2 concentration.Age-stage two-sex life table of A.hygrophila F5 generations feeding on A.philoxeroides growing in different CO2 concentrations(420 ppm,750 ppm)were constructed.The results showed that,as the CO2 concentration increasing,the development was shortened,the oviposition period was lengthened,and the fecundity increased of A.hygrophila feeding on A.philoxeroides growing in correspond environment.In the same CO2 concentration(420 ppm)condition,the oviposition period and fecundity of F5 were less than F1,but the development duration was lengthened.The R0 and r of F5 in 750 ppm CO2 concentration condition were also less than F1,but higher than that of F5 in 420 ppm CO2 concentration,and in the 420 ppm CO2 concentration condition,the net reproductive rate(R0),intrinsic rate of increase(r)of F5 were less than F1,The finite rate of increase(λ)and mean generation time(T)were no significantly different.The fertile female ratio at higher concentration was significantly higher than those at lower concentration.In this paper,we determined the transcriptional groups of female and male adults of feeding on A.philoxeroides growing under different concentrations of CO2(420 ppm F1,420 ppm F5,750 ppm F5),the total of 119.43 Gb data were obtained using BGISEQ-500 platform.55,351 Unigene were got,the total length,average length,N50 and content of GC were 84,000 065 bp,1,545 bp,2,866 bp and 35.71%respectively,the Q20 of each sample was greater than 97%and Q30 was greater than 88%.And then the Unigene was annotated in the Nr,Nt,SwissProt,KEGG,GO,KOG and Interpro databases respectively.There 33,141(NR:60.98%),10,026(NT:18.45%),24,560(SwissProt:45.19%),24,127(KOG:44.39%),25,608(KEGG:47.12%),13,390(GO:24.64%)and 25,053(InterProp:46.09%)unigenes were annotated.There 357,147 CDSs were detected by Transdecoder,6,208 SSRs of 5,123 Unigene were detected,and 5,232 encoding transcription factors of Unigene were predicted at the same time.The DEG were detected,and the analysis of functional analysis of GO and Pathway of DEG were detected.The analysis results of GO function showed that the up and down of cell process of Biological Process,membrane of Cellular Component and binding of Molecular Function of DEG were the most.The results of Pathway function Analysis of DEG were showed that,the increase of CO2 concentration had the greatest effect on the Metabolic Pathways and Ribosome of the enrichment pathway of DEG in female and male adults of A.hygyophila.In this paper,the indirect response of A.philoxeroides to the increase of atmospheric CO2 concentration was studied,to analyze the change of growing and development of A.philoxeroides by population ecology and molecular level,in order to provide the basis of relevant theoretical for biological control of A.philoxeroides under the condition of increasing of global atmospheric CO2 concentration in the future. |
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