Preliminary Function Analysis Of Cassava MinD Gene Related To Plastid Division

The synthesis and storage of starch occurs in the amyloid.The morphology and quantity of amyloid are affected by plastid division.The Min system is an important regulatory factor in plastid division.The MinD protein is an important part of the Min system.MinD genes are widespread from lower prokaryotes to higher plants.The MinD protein regulates cell division in prokaryotic cells and regulates plastid division in plant cells.In this study,the MinD gene was cloned from cassava and named MeMinD.Bioinformatics analysis showed that MeMinD protein is a typical member of MinD family.MeMinD protein has the same or similar structure as EcMinD protein.Homologous sequence alignment showed that the homology between MeMinD and EcMinD protein reached 32.05%.The evolutionary analysis of MinD protein showed that the cassava and Populus tomentosa had the closest phylogenetic relationship.The overexpressed MeMinD:EGFP in wild-type E.coli BL21(DE3)shown that MeMinD still functions in bacterial,which suggested evolutionary conservation of MeMinD.The MeMinD protein is located in plastid,as revealed by a transient expression assay using a full-length MeMinD protein fused to enhanced green fluorescent protein(EGFP).Studies on different spatial and temporal expression patterns of MeMinD in cassava indicated that it expressed much higher in young leaf than in non-photosynthetic,and more active at the later expanding stage and the tuber mature stages.Plantlets were obtained from explants of cassava in South China 8.The somatic embryos were induced by axillary buds of tissue culture seedlings,and the somatic embryonic cotyledons were further induced to somatic cotyledons serve as transforming receptors.We constructed the pVKH-CaMV35S-MeftsZ3-pA and pVKH-CaMV35S-MeMinD-pA recombinant engineering bacteria.Recombinant engineered bacteria transformed the somatic embryos of the cotyledons.After screening and cultivation,young shoots containing the pVKH-CaMV35S-MeftsZ3-pA recombinant vector were obtained.In this study,preliminary functional analysis of the cassava MeMinD gene was performed,which provided a theoretical basis for the application of MeMinD gene in the improvement of cassava starch quality.