Effects Of Dietary Different Supplement Levels Of Seabuckthorn Flavones On Growth Performance,Digestion Metabolism,Rumen Fermentation And Blood Index In Sheep

The objective of this study was to evaluate the effect of seabuckthorn flavones on the growth performance,rumen fermentation,and digestion and metabolism of fattening sheep(0、0.1%、0.2%、0.3%、0.4%和0.5%),in vitro gas production and nylon bag method were used to evaluate the nutritional value of diets with different levels of seabuckthorn flavones.Then,36 Dupo × Small Tail Han sheep hybrid male lambs were used to conduct feeding and digestion and metabolism experiments.The results showed as follows: 1)seabuckthorn flavones significantly increased the gas production in each time point(P<0.05),and the gas production reached the maximum value when the supplementation of seabuckthorn flavones was 0.5%;the content of microbial protein was significantly increased with the increase of supplementation of seabuckthorn flavones(P<0.05).Compared with the control group,seabuckthorn flavones significantly reduced the concentration of methane(P<0.05);the contents of acetic acid was significantly reduced,propionic acid TVFA were significantly increased(P<0.05).The supplementation of0.4% and 0.5% seabuckthorn flavones reduced Ruminococcus albus,Ruminococcus flavefaciens,Protozoa,Methanogens and total bacterial(P<0.05)compared with other groups.2)The average daily gain of the0.1% and 0.3% groups was significantly higher than that of the control group(P<0.05);the feed efficiency of each group was significantly higher than that of the control group(P <0.05);The apparent digestibility of DM,NDF and ADF in the 0.3% and 0.5% group was higher than that in the control group(P<0.05);the excretion of MCP in the 0.1%,0.3% and 0.5% groups was significantly higher than that in the control group(P <0.05).3)The TG and HDL in the 3% and 0.5% groups were significantly higher than the control group(P <0.05);the LDL in the 0.3% and 0.5% groups was significantly lower than that in the control group(P<0.05);the T-AOC,SOD and CAT of the 0.3% and 0.5% groups was significantly higher than the control group(P<0.05),the MAD of the 0.3% and 0.5% groups was significantly lower than the control group(P<0.05);the Ig G and Ig M in the 0.3% and 0.5% groups were significantly higher than the control group(P<0.05);the IL-2 and IL-6 in the 0.3% and 0.5% groups were significantly lower than the control group(P<0.05).4)The acetic acid and acetic acid/propionic in the 0.5% groups were significantly lower than the control group(P<0.05),The propionic acid and total volatile fatty acids in the 0.3% and0.5% groups were significantly higher than the control group(P<0.05);The NH3-N in the 0.3% and 0.5%group was significantly lower than that in the control group and 0.1% group(P<0.05);the hydroxymethylcellulase and xylanase in the 0.1%,0.3% and 0.5% groups were significantly higher than those in the control group(P<0.05);0.3% and 0.5% group R.albus,R.flavefaciens and F.succinogens were significantly higher than the control group(P<0.05);the 0.3% and 0.5% groups of Protozoan and Methanogens were significantly lower than those of the control group(P<0.05);In conclusion,the addition of seabuckthorn flavones to the diet can improve the fermentation in vitro and increase the degradation rate of nutrients.Feeding experiment showed seabuckthorn flavones can improve the average daily weight gain,feed conversion rates,improved the antioxidant and immune abilities and improved rumen fermentation.Therefore,seabuckthorn flavones can be used as a new feed additive for fattening lambs,and the optimal proportion was 0.3% under the condition of this study.