| Fat deposition affects metabolism and energy storage in animals,which is one of the hot spots and problems to be solved.Adipocyte differentiation and metabolism in animals is a complex process.Under the influence of many factors,coding genes and non-coding genes regulate each other and form a coordinated network.The analysis of animal adipose tissue metabolism regulation network has important reference value and guiding significance for improving the intramuscular fat content of beef cattle in China,miRNA plays an important role in regulating fat development and studying its regulation on adipocyte differentiation and metabolism,which is helpful to reveal the molecular mechanism of meat production traits and improve beef cattle heredity for theoretical basis.This study used bovine specific miRNA,intron miRNA and host genes to regulate adipocyte differentiation and metabolism as preliminary screening conditions.Bioinformatics analysis,tissue expression profiling and double luciferase reporter system were used to verify the target gene in order to explore its effect on intramuscular deposition in beef cattle.The results showed that miR-2439-5p is a bovine specific miRNA,host gene MRTFA may promote the development of adipose progenitor cells to mature adipocytes,and the production of miR-2439-3p may have a special regulatory mechanism for myogenic differentiation of skeletal muscle cells in beef cattle and dairy cows.miR-2439-5p may be involved in bovine adipocyte differentiation and metabolic regulation.miR-2439-5p was highly expressed in heart,lung,inguinal fat and back fat of Wagyu at 28 months of age,and the expression of miR-2439-5p in back fat was the highest,which was significantly different from that of pericardial fat,liver and spleen(P<0.05).miR-2439-5p in Qinchuan cattle at 28 months old(P<0.05).SMAD3 gene was the target gene of miR-2439-5p.In addition,the successful isolation,culture and induced differentiation of bovine intramuscular preadipocytes provided a good cell material for subsequent functional verification tests. |
