| Yellow River carp(Cyprinus carpio haematopterus Temminck et Schlegel)is an important cultured fish in the Yellow River Basin of China.Because of its advantages of fast growth,strong resistance to adversity and delicious taste,Yellow River carp is deeply loved by farmers and consumers.However,in the past few decades,with the rapid development of the Yellow River carp breeding industr y,the growth and stress resistance of the Yellow River carp have been declining,so it is urgent to select new varieties with high yield and stress resistance t o promote the healthy and sustainable development of the Yellow River carp industry.In this study,we established a Yellow River carp family and injected it with pit markers when its body length was about 10 cm.The body weight,length,height and thickne ss of the famil y were measured at the age of 4 months,9 months,13 months and 17 months,respectively.After the age of 17 months,we cut the fins to construct the genetic linkage map and locate the conditional and unconditional QT L of growth traits of Ye llow R iver carp by Super-GBS technolog y.The main results are as follows :(1)Construction of genetic linkage map of Yellow River carp: the high-density genetic map of Yellow River carp was constructed by Joinmap5 software.After screening and filtering,10286 SNP markers were used to construct the genetic map of Yellow River carp.There are 50 linkage groups in the integrated map of Yellow River carp,the total length of the map is 3474.01 c M,the average distance between markers is 0.35 c M,the distance between markers is from 0.19 c M to 0.62 c M,the average length of the linkage group is 69.48 cm,and the length of the linkage group is from 52.10 c M to 133.70 c M.The genetic map of male and female also has high marker density.The female genetic map consists of 6702 markers.The total length of the map is 3003.984 c M,the average distance between markers is 0.49 c M,the distance between markers is from 0.20 c M to 0.83 c M,the average length of the linkage group is 60 c M,and the length of the linkage group is from 45.81 c M to 136.97 c M.The male genetic map consists of 5478 SNP markers.The total length of the map is 2899.883 c M,the average distance between markers is 0.56 c M,the average distance between markers is from 0.25 c M to 0.95 c M,the average length of the linkage group is 57.99 c M,and the length of the linkage group is from 34.99 c M to 79.87 c M.(2)Location and analysis of QT Ls related to body weight of Yellow River carp.A total of 116 unconditional QT Ls were detected at 4 months,13 months and 17 months of age and distributed in different linkage groups.Four unconditional QTLs were detected at the same time in three periods,the interpretable phenotypic variation rate was between 0.20% and 4.64%,27 unconditional QT Ls were detected at two periods,the explainable phenotypic variation rate was between 0.01% and 48.92%,the remaining 85 unconditional QT Ls were only detected at a single period.A total of 103 conditional QT Ls related to body weight were detected,of which 7 loci could be detected in 3 periods,with the interpretable phenotypic variation rate between 0.02% and 50.29%,29 conditional QT Ls were detected in 2 periods at the same time,and the other 67 loci were only detected in a certain period,with the explainable phenotypic variation rate between 0.01 % and 87.48%.(3)Location and analysis of QT Ls related to body length of Yellow River carp.A total of 45 unconditioned QTLs were detected in three different measurement periods.Four unconditioned QT Ls were detected in the 39 th linkage group at the sa me time in three periods,and the explainable phenotypic variation was between 6.74-10.29%;the remaining 42 unconditioned QTLs were detected in one or two periods respectively;174 conditional QTLs affecting body length were detected in total,two of whic h were detected in three periods,and the explainable phenotypic variation was only 1.01-9.21%,and the other 172 conditional QT Ls were detected in one or two periods,respectively.The rate of interpretable phenotypic variation was between 0.01 % and 96.4%.(4)Location and analysis of QTLs related to body height in Yellow River carp.A total of 149 unconditional QT Ls were detected in three different measurement periods,two of them were detected in two periods at the same time,with the highest explainable phenotypic variation rate of 11.84%;the other 147 unconditional QT Ls were detected in one period,with the explainable phenotypic variation rate between 0.01% and 94.63%;171 conditional QTLs affecting body height were detected,one of which was detec ted in three periods.The results showed that the rate of explainable phenotypic variation was only 0.46%,and the other 170 conditional QT Ls were detected in 1 or 2 periods respectively.The rate of explainable phenotypic variation was between 0.01% and 81.16%.(5)Location and analysis of QT Ls related to body thickness in Yellow River carp.A total of 46 unconditional QTLs were detected in three different measurement periods.Only three unconditional QTLs were detected in two periods at the same time,w ith rate of explainable phenotypic variation was between 4.72 % and 9.03%;the remaining 43 unconditional QT Ls were detected in one period respectively;a total of 41 conditional QT Ls affecting body thickness were detected,only one conditional QT L was dete cted in two periods,with the rate of explainable phenotypic variation was between 0.67% and 1.45%,the other 400 conditional QT Ls were detected in one period,and the highest explainable phenotypic variation rate was 79.42%.In conclusion,a high-density genetic linkage map of Yellow River carp was constructed with 50 linkage groups and 10286 SNP markers.A total of 708 conditional and non conditional QT Ls were identified,including 152,193,280 and 83 QTLs related to body weight,length,height and thick ness,respectively.It was found that the QTLs affecting the growth of Yellow River carp in different periods were quite different.Some loci existed in multiple periods at the same time,and some loci only existed in a certain period.The results are help ful to further analyze the growth and development mechanism of Yellow R iver carp and provide basis for molecular marker assisted breeding of growth traits of Yellow River carp. |
PDF Full Text Request