Analysis Of The Differences In Disease Resistance Between JG21 And G1 Based On WGCNA And Whole Genome Resequencing In Foxtail Millet

Downy mildew of foxtail millet is a oomycetes disease caused by Sclerospora graminicola.It occurs in the whole growth stage of foxtail millet and presents different symptoms.In serious cases,it can greatly reduce the yield and quality.At present,there are few studies on the pathogenesis of downy mildew of millet.Therefore,this study intends to analyze and compare transcriptometric data and re-sequencing data of Jingu 21(highly susceptible)and G1(highly resistant)at the seedling stage through bioinformatics methods,so as to preliminarily explain the molecular mechanism of the bacteria resistant to downy mildew of foxtail millet,and provide a theoretical basis for the resistance breeding of foxtail millet.The main research contents are as follows:(1)Family gene analysis of foxtail millet LRR-RLK mainly includes identification and gene location analysis of foxtail millet LRR-RLK genes,structural analysis of genes,analysis of conserved motifs and conserved domains of genes,phylogenetic evolution and collinearity analysis between genomes.The results showed that there were 250 LRR-RLK family genes,and most of the l RR-RLK family genes were distributed on chromosome 8.There are 10 conserved structural domain,and the family is divided into 3 categories in phylogenetic tree.Gene structure analysis shows that Motif1 and Motif3 are the most conserved than other motifs.There were 18 LRR-RLK family genes collinearity with foxtail grass,indicating that this family gene may play a important role in disease resistance.(2)WGCNA analysis was performed on the differentially expressed genes of 36 transcriptomics from the seedling stage of inoculated resistant cultivars(Jingu 21 and G1)and LRR-RLK family resistant genes.The module with the most differentially expressed genes was identified,and analysis showed that the seedlings of Jingu 21 and G1 were infected by the S.graminicolaof foxtail millet.A total of 17077 differentially expressed genes were divided into 44 modules,among which 3 modules related to disease resistance were analyzed by network visualization,and the genes in the selected specific modules were analyzed by GO enrichment in Tbtools.These module genes were respectively enriched into resistant pathways such as defense response,response to stimulation,programmed cell death,calcium ion binding and anabolic metabolism.Finally,12 hub genes,Seita.9G071200、Seita.9G456400、Seita.7G183700、Seita.8G148400、Seita.9G307500、Seita.9G115300、Seita.9G090900、Seita.9G101200、Seita.2G372300、Seita.9G485400、Seita.6G000400、Seita.5G323800,were extracted,Seita.9G307500、Seita.9G456400、Seita.9G163300、Seita.9G203800、Seita.9G386400、Seita.9G273500、Seita.9G525900、Seita.9G101200 are the predicted candidate genes.These genes may play a very important role in disease resistance in foxtail millet.(3)Based on the resequencing data of highly susceptible Jingu 21 and highly resistant G1,and taking Setaria_italica_v2.2 as the reference genome,the differences of the resequencing data of the two varieties were analyzed: data evaluation,comparison with the reference genome,mutation detection,etc.The clean data was 33.55 Gbp and Q30 was 93.31%,and the comparison rate with the reference genome was 98.11%.Through resequencing analysis,it was found that various mutation sites,non-synonymous mutations and mutation genes of G1 species were more than those of Jingu 21 species,which provided a part of sequence basis for studying the difference between Jingu 21 and G1 species and studying the disease resistance of Jingu 21.To sum up,The bioinformatics analysis of 250 l RR-RLK family genes provided a theoretical basis for further research,identification and functional verification of resistance genes in foxtail millet.Through WGCNA analysis,we discovered candidate genes related to disease resistance of foxtail millet,which provided an important theoretical basis for further research on resistance mechanism of foxtail millet.This research through the valley of bioinformatics methods to explore the Jingu21 and G1 two foxtail millet varieties in homozygous mutations,non synonymous mutations,heterozygous mutation,frameshift mutations,SV structure variation of insertions and deletions and exon variation,as well as the analysis of the catalytic activity in the enrichment cells process,the immune system,metabolism,response to stimuli,extracellular region,film part and nucleic acid in combination with the activity of transcription factors such as entry has a lot of difference,these differences is probably the cause of two different varieties resistance to sense mechanism.