| Soybean（Glycine max L.）,as an important oil and economic crop in the world,occupies a very important position in agricultural production.Soybean is a short-day crop,and its growth and development are very sensitive to photoperiod.Short-day light can promote flowering,while long-day light inhibits flower bud growth.Therefore,soybean is considered as an important model plant in the early study of photoperiod pathway.This feature has greatly hindered the adaptability of soybean varieties to different regions,which is not conducive to the promotion of good varieties.The flowering and maturity stages of soybean are important a gronomic traits.Due to the narrow adaptation range of each soybean variety,we need to cultivate soybean varieties with different photoperiod responses to adapt to different natural environment and ecological conditions.In order to effectively reduce the sensitivity of soybean under different light treatments and reduce the pressure of breeding new varieties of soybean,it is more and more important to excavate new photoperiod genes controlling flowering and maturity of soybean and clarify their molecular mechanism.Soybean GmRAV gene contains two domains,AP2 and B3,which are important genes for photoperiod regulation.In this study,GmRAV gene was cloned to construct plant overexpression vector for transformation of soybean,and the biological function of GmRAV gene in soybean was studied;semi-quantitative detection of the expression level of GmRAV protein under long and short days by western blot;RNA-seq technology performed transcriptome sequencing on GmRAV gene overexpression plants and wild-type soybean plants to obtain differentially expressed genes related to flowering and growth.The main experimental results are as follows:（1）Six T0 generation GmRAV transgenic positive soybean plants were obtained by genetic transformation.Under long-day and short-day conditions,three different lines of T3 generation GmRAV transgenic soybean plants showed lower plant height and late flowering compared with wild type plants.（2）Semi-quantitative analysis by western blot found that the expression level of GmRAV protein under short-day light was higher than that under long-day light,it shows that the expression of GmRAV protein can be better enriched under short-day conditions.（3）Transcriptome sequencing analysis of GmRAV gene overexpression plants and wild-type soybean plants revealed a total of 10627 differentially expressed genes,including 5281 up-regulated genes and 5346 down-regulated genes.Among them,differentially expressed up-regulated genes and down-regulated genes are involved in biological processe and molecular function.（4）Real-time fluorescent quantitative PCR studies have shown that the GmRAV gene acts downstream of the E3E4 gene to inhibit flowering by inhibiting the expression of the Gm FT2a gene or promoting the expression of the Gm PRR37 gene. |
