| Short-term or long-term high temperature stress can cause a series of morphological,physiological and biochemical changes of plants,affecting the growth and development of plants,and even lead to a serious decline in economic output.Most previous studies have focused on the regulation of heat stress responses with genes or transcription factors（such as heat stress transcription factors that regulate the expression of heat-responsive genes）and further influence the accumulation of heat shock proteins to affect plant heat tolerance.Sex.However,recent studies have found that more and more emerging non-coding RNAs play an important role in thermal reactions,and their regulatory mechanisms are revealed in plants.Studies have shown that RNA can be mutually regulated by competition with a common microRNA response element that constitutes a competitive endogenous RNA（ceRNA）.The theory of competing endogenous RNAs has been demonstrated and is now widely accepted.However,a comprehensive identification and analysis of lncRNAs and circRNAs as ceRNAs have not yet been completed in cucumber exposed to high-temperature stress.Using high-throughput sequencing and bioinformatics analysis,we will construct ceRNA networks of lncRNAs,circRNAs,miRNAs,and mRNAs,and analyze their potential regulatory effects by GO and KEGG.This study is to conduct a full transcriptomic analysis of cucumber response to high temperature stress,and to integrate the potential ceRNA function of lncRNAs/circRNAs during the high temperature stress of cucumber,and to provide a basis for studying the potential function of lncRNAs/circRNAs in response to high temperature stress.In this study,two high-throughput sequencing libraries of control group and high temperature treatment group were constructed with ’Improved Jinchun No.2’.The mRNAs,lncRNAs,circRNAs and miRNAs in response to high temperature stress were isolated and identified by sequencing technology.The ceRNA networks of lncRNAs,circRNAs,miRNAs and mRNAs were constructed by bioinformatics analysis,and their potential regulation was analyzed by GO and KEGG.The main findings of the paper are as follows:1.Three thousand two hundred six hundred sixty three mRNAs,2085 lncRNAs,2477 circRNAs,and 348 differentially expressed miRNAs were identified using RNA sequencing technology.Six miRNAs（5 known and 1 new miRNA）and 8 lncRNAs in response to high temperature stress were selected for qRT-PCR to confirm their expression profiles,and the trend was consistent with high-throughput sequencing results,indicating that the sequencing results were reliable.2.By analyzing the cis effect of lncRNA,we constructed a lncRNA-mRNA co-expression network.Based on this result,it was found that lncRNA may be involved in stimulating response by regulating gene expression.3.Patmatch software was used to predict the potential function of lncRNAs and circRNAs as ceRNAs,and GO and KEGG analyses were performed.A total of 18 lncRNAs and 7 circRNAs were predicted to bind to 114 differentially expressed miRNAs and compete with 359 mRNAs for miRNA binding sites.These mRNAs are predicted to be involved in signaling pathways such as plant hormone signaling,plant-pathogen interaction,and glutathione metabolism.Among them,TCONS00031790,TCONS00014332,TCONS00014717,TCONS00005674,novelcirc001543,and novelcirc000876 may interact with miR9748 and participate in response to high temperature stress through the plant hormone signaling pathway.Moreover,IAA and ACC levels decreased in the high-temperature treatment group,indicating that IAA and ethylene signaling might be involved in response to high-temperature stress.In this study,a full transcriptomic analysis of cucumber response to high temperature stress was carried out,and the potential ceRNA function of lncRNAs/circRNAs was integrated in high temperature stress of cucumber,which provided a basis for studying the potential function and mechanisms of lncRNAs/circRNAs in response to high temperature stress. |
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