| Wheat(Triticum aestivum L.)has an important strategic position in national economic development and food security.With the continuous improvement of wheat production,the change of farming system and the warming of the global climate,the fungal disease of wheat stem base caused by Rhizoctonia cerealis and Rhizoctonia solani has become an influence.One of the major diseases of wheat production in China has been included in the list of "major" pests and diseases by the National Agricultural Technology Extension Service Center of the Ministry of Agriculture.The disease mainly occurs in the middle and lower reaches of the Yangtze River and the Huanghuai wheat area in China.The light yield loss is 5%-10%,and the heavy loss yield is 20-40%.At present,no varieties that are immune to sharp eyespot are found in wheat,and most of the popular varieties that are grown in large areas are susceptible to sharp eyespot,and it is necessary to discover the genes for resistance to sharp eyespot.In this study,124 F2:8 generation Recombinant inbred lines(RIL)populations of Soru#1×Naxos combination were constructed by single-grain transmission method,respectively,in the field of greenhouses and greenhouses for three consecutive years in 2016-2019.The pathogenic strain R0301 was induced by wheat inoculation method and toothpick insertion method to identify wheat sharp eyespot,and the statistical RIL population disease index(DI)was identified.Using the Illumina iSelect 90K chip technology to screen the RIL population polymorphism SNP markers,a 21-chromosome genetic linkage map containing 1,267 marker loci was constructed,covering a full-length genome of 2437.78 cM with an average density of 1.92 cM/Locus.Based on the disease index(DI)and genetic linkage map,using the ICIM function of the software IciMapping 4.1,two QTLs for resistance to sharp eyespot were found,located on chromosomes 2B and 5 A,named Qse.jaas-2B and Qse.jaas-5A.Qse.jaas-2B and Qse.jaas-5A were detected in 4 environments and explained 7.59-10.86%and 10.90-20.53%phenotypic variation,respectively.In addition,the correlation between the plant height,heading stage and wall thickness phenotype values and related QTLs of the RIL population and the QTL for resistance to sharp eyespot were analyzed,and it was found that Qse.jaas-2B was closely related to the heading stage of RIL,and The targeted heading-related QTL(Qhd.jaas-2B)and Qse.jaas-2B overlap on chromosome 2B.Qse.jaas-5A has no linkage relationship with main agronomic traits,and its detection is stable.The markers on both sides are closely linked and can be used for molecular marker-assisted selection breeding.Twelve Wheat-Dvillosum whole arm translocation lines were identified for resistance to sharp eyespot,and it was found that the disease index of Wheat-D.villosum translocation line T2DS-2VL,T4DL-4VS·and T5DL·5VS was significantly lower than that of background parents.Furthermore,the T2DS·2VL/dwarf resistant 58 BC1F2 population was inoculated and identified,and the disease:index of the translocation chromosome was also significantly lower than that of the non-exogenous chromosome.It is speculated that the 2VL of the hairy wheat may carry the resistance to the sharp eyespot disease gene.The resistance effect and the value of breeding utilization need to be analyzed in depth using the near system. |
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