| This experiment aims to investigate the optimal dosage of fermented TCM compound preparation in the diet,and its effects on growth performance and immune function of weaned piglets.Experiment I:This experiment mainly aims to determine the optimal dosage of fermented TCM compound preparation through the effects of fermented TCM compound preparation at different doses on the growth performance of weaned piglets.A total of 120 weaned piglets(male:female,1:1)were selected and randomly divided into four treatment groups,with three replicates in each treatment and 10 piglets in each replicate.The four treatment groups were the control group,0.1%,0.2%and 0.3%fermented TCM compound preparation group.The control group was fed with a basic diet,while the 0.1%,0.2%and 0.3%fermented TCM compound preparation groups were fed with 0.1%,0.2%and 0.3%fermented TCM compound preparation in addition to the basic diet,the experiment lasted for 23 days.The experiment results showed that:(1)The levels of ADG and ADFI in the 0.2%group were significantly higher than those in the 0.1%and control groups(P<0.05)but not significantly different from the 0.3%group(P>0.05).Compared with the control group,the average daily feed intakes in groups added with 0.1%,0.2%,and 0.3%compound preparations of fermented TCM increased by 2.61%,5.49%,and 3.85%,respectively.The 0.2%group had the highest average daily feed intake.(2)The feed to gain ratio(F/G)in the 0.2%group was significantly lower than those in the 0.1%and control groups(P<0.05)but not significantly different from the 0.3%group(P>0.05).(3)The diarrhea rate in the 0.2%group was significantly lower than those in the 0.1%and control groups(P<0.05)but not significantly different from the 0.3%group(P>0.05).A comprehensive analysis of the results of the four treatments demonstrated that the optimal dosage of the above fermented TCM compound preparation was 0.2%.Experiment Ⅱ:This experiment mainly aims to investigate the effects of fermented TCM compound preparation on the growth performance and immune function of weaned piglets.A total of 120 weaned piglets(male:female,1:1)were selected and randomly divided into four treatment groups,with three replicates in each treatment and 10 piglets in each replicate.The four treatment groups were the control group,antibiotic group,unfermented group and fermented group.The control group was fed a basic diet,while the antibiotic group,unfermented group and fermented group were fed with 75 g/t chlortetracycline+40 g/t zinc bacitracin,0.2%unfermented TCM compound preparation and 0.2%fermented TCM compound preparation,respectively,in addition to the basic diet.The experiment results showed that:(1)As for growth performance,the average daily gain in the fermented group was significantly higher than those of the unfermented and control groups(P<0.05)but not significantly different from the antibiotic group(P>0.05).The average daily feed intake in the fermented group was significantly higher than that in other groups(P<0.05).The F/G in the fermented group was significantly lower than those in the unfermented and control groups(P<0.05)but not significantly different from the antibiotic group(P>0.05).There was no significant difference in diarrhea rate among the fermented,unfermented,and antibiotic groups(P>0.05),and they were significantly lower than the control group(P<0.05).(2)As for immune function,the IL-2,IL-4,and IFN-γ levels in the pig serum of the fermented group were significantly higher than those of the antibiotic,unfermented,and control groups(P<0.05).The serum TNF-α level of the fermented group was significantly higher than those of the antibiotic and control groups(P<0.05)but not significantly different from the unfermented group(P>0.05).The serum IgG level of the fermented group was significantly higher than those of the antibiotic,unfermented,and control groups(P<0.05).The CD4+T cell level in the peripheral blood of the fermented group was significantly higher than those of the antibiotic and control groups(P<0.05)but not significantly different from the unfermented group(P>0.05).The CD8+T cell level in the peripheral blood of the fermented group was significantly higher than that of the control group(P<0.05)but not significantly different from the unfermented and antibiotic groups(P>0.05).The CD4+/CD8+ratio in the peripheral blood of the fermented group was significantly higher than that of the antibiotic group(P<0.05)but not significantly different from the unfermented and control groups(P>0.05).Experiment Ⅲ:This experiment mainly aims to investigate the effects of fermented TCM compound preparation on microbial flora in feces of weaned piglets.A total of 40 weaned piglets(male:female,1:1)were selected and randomly divided into five treatment groups,with four replicates in each treatment and 2 piglets in each replicate.The five treatment groups were the control group,antibiotic group,Compound probiotics group,unfermented group and fermented group.The control group was fed a basic diet,while the antibiotic group,Compound probiotics group,unfermented group and fermented group were fed with 75 g/t chlortetracycline,250 g/t Compound probiotics,0.2%unfermented TCM compound preparation and 0.2%fermented TCM compound preparation,respectively,in addition to the basic diet.The experiment results showed that:(1)The fecal Lactobacillus number of the fermented and unfermented groups were significantly higher than those of the control and antibiotic groups(P<0.05),and significantly lower than those of the compound probiotics group(P<0.05).(2)The fecal Bifidobacterium number of the fermented group and compound probiotics group had no significant difference(P>0.05),but was significantly higher than those of the unfermented,control and antibiotic groups(P<0.05).(3)The fecal E.coli number of the fermented and antibiotic groups had no significant difference(P>0.05),and were significantly lower than those of the unfermented,compound probiotics and control groups(P<0.05).In summary,the fermented TCM compound preparations used in this experiment could effectively improve the daily gain of weaned piglets and reduce the diarrhea rate,with effects equivalent to antibiotics.It could significantly improve the immune function of weaned piglets and worked better than the unfermented TCM compound preparations and antibiotics.It could significantly increase the number of Lactobacillus and Bifidobacterium in feces,and reduce the number of E.coli in feces.The optimal dosage is 0.2%. |
PDF Full Text Request