| Reactive Oxygen Species(ROS)is an important signal molecule in plants,which are involved in the regulation of plant growth and development,biotic and abiotic stress and cell death.Wheat(Triticum aestivum L.)is an important food crops in the world.Up to now,the molecular mechanism of ROS regulation is not clear in wheat.ROS synthesized by respiratory burst oxidase is the main source of ROS in plants.In this study,the TaRBOHs genes were identified using the bioinformatics technology in wheat.The expression level of TaRBOHF1 s was relatively higher in the shoot apical meristem and root apical meristem.So,the function of TaRBOHF1 s was researched.The main results are as follows:There are 43 TaRBOHs genes in wheat genome.Through the analyses of gene structure,conserved domain and the alignment of amino acid sequence,it was showed that TaRBOH proteins were highly conserved in wheat.TaRBOHF1 s have three alleles of TaRBOHF1-A,TaRBOHF1-B,and TaRBOHF1-D.Amino acid sequence alignment showed that the homology of TaRBOHF1-A,TaRBOHF1-B and TaRBOHF1-D is about 92%.Transient expression results showed that TaRBOHF1 s proteins were located in the nucleus and cytoplasm.The quantitative PCR analysis showed that TaRBOHF1 s could been detected in all tissues of wheat the expression level of TaRBOHF1-A is highest.Compared with wild type plants,TaRBOHF1s-RNAi transgenic wheat plants had more tillers and shorten root.The qualitative and quantitative determination found that ROS contents were significantly reduced in TaRBOHF1s-RNAi transgenic wheat plants.To understand the mechanism of TaRBOHF1 s,we performed the transcriptome analysis of TaRBOHF1s-RNAi root apical meristem was performed.It was found that the genes involved in the environmental and hormonal signaling pathways were enriched.In conclusion,TaRBOHF1 s may regulate the ntiller number and root development in wheat.This study could provide reference for the regulation of tiller development in wheat. |
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