| Soil salinization can lead to a decline in crop yield and quality.Metabolites are the result of gene transcription under the regulation of internal and external factors in plants,and are the material basis for plant phenotypes.In order to study and analyze the regulation mechanism of salt-tolerant molecules,this study used common bean roots treated with sodium chloride(Na Cl)as the material.Based on morphological analysis,transcriptomics and metabonomics analysis were used to study the response of common bean to Na Cl.The main research results are as follows:1.Under salt stress,the germination rate,germination potential,germination index,vigor index and root length,surface area,average diameter and volume of black kidney bean and milk flower kidney bean were measured.It was found that each index was lower than that of its own control.The range of change was higher than that of black kidney beans,indicating that black kidney beans are more salt-tolerant.2.In this study,based on the phenotype,with 0 h as the control,transcriptome analysis was performed on the roots of black kidney bean at 3 h,6 h,12 h,and 24 h under salt stress.The two treatment groups were compared to obtain 1470,2054,and 2054,respectively.2366 and 1680 differentially expressed genes(DEGs).Further analysis revealed that 510 DEGs were co-expressed.KEGG analysis found that phenylpropane biosynthesis,flavonoid biosynthesis,phenylalanine metabolism,and plant hormone signal transduction pathways were significantly enriched.set.Among the 510 DEGs,90 transcription factors are classified into 28 gene families,including MYB,NAC,AP2/ERF,b HLH,b ZIP,WRKY and other transcription factor families related to salt stress.3.Metabolomics analysis found that compared with the control at 0 h,differential expression analysis was performed at 12 h and 24 h under salt stress,and 125 and 141 differentially expressed metabolites(DGMs)were detected respectively,of which 77 DGMs were between treatment groups.There are 16 ingredients,including flavonoids(flavonoids,flavonols,flavanols,isoflavones,anthocyanins)(45.5%),phenylpropanoids(9.1%),amino acids and derivatives(6.5%),etc.ingredient.KEGG enrichment analysis found significant enrichment to phenylpropane biosynthesis,flavonoid biosynthesis,flavonoids and flavonol biosynthesis.Among metabolites related to flavonoid synthesis,coumarin-Co A focuses on the synthesis of quercetin and other substances,and caffeoyl-Co A focuses on the synthesis of lignin.Correlation analysis showed that quercetin was significantly positively correlated with L-phenylalanine and p-coumaric acid,and was related to trans-cinnamic acid,naringin chalcone,naringenin,dihydrokaempferol,and apigenin there was a significant or extremely significant negative correlation between saccharol and saccharol.These precursor metabolites are closely related to the synthesis of quercetin,and their content determines the accumulation of quercetin.4.Transcriptome and metabolome analysis screened 10 types of flavonoid synthesis-related enzyme genes and measured their enzyme activity.It was found that gene expression and enzyme activity were closely related to the content of polyphenols,flavonoids,and total flavonoids.PAL gene expression is positively correlated with enzyme activity,C4 H,CHS,CHI,FLS,F3’H gene expression is negatively correlated with enzyme activity,4CL,POD,DFR,CCAOMT gene expression is correlated with total phenols,flavonoids and total flavonoids are extremely negatively correlated,and positively correlated with enzyme activity,further indicating that there is a complete pathway for the synthesis of flavonoid metabolites in black kidney beans.In summary,this study is based on the results of phenotype,transcriptome and metabolome data,and found that common bean needs more gene-regulated enzyme expression to catalyze intermediate products and synthesize more flavonoid metabolites when resisting adversity stress.Thereby enhancing the tolerance of kidney beans. |
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