Construction And Analysis For The Single-cell Transcriptome Atlas Of Worker And Queen’s Brain In Honey Bee,Apis Mellifera

Apis mellifera is an important economic insect,its unique phenomenon of caste differentiation and the mechanism of labour division have always been a hot research topic.These typical behaviors of Apis mellifera make them an important model for studying the behavior regulation of insect brains.In recent years,single-cell RNA sequencing(sc RNA-seq)technology has become a popular research method for studying complex tissues(such as brain tissue).Scientists have completed the construction of brain single-cell transcriptomic atlas of Drosophila,but the study of brain single-cell transcriptomic atlas of Apis mellifera is still a scanty.In this study,10 x scRNA-seq technique was used to construct the brain transcriptomic atlas of Apis mellifera worker and queen,and clustering analysis is carried out on the detected cells,with referring to the marker genes in Drosophila single-cell transcriptome sequencing.The main research results are as follows:1.Correlation analysis between single cell transcriptome sequencing data and common transcriptome sequencing data showed a good linear correlation,verifying that the sequencing results of single cell transcriptome were relatively complete and accurate.2.Construction for the single-cell transcriptome atlas of brain of Apis mellifera,and according to the marker gene known in Drosophila,the cells in the brain of Apis mellifera were initially divided into three types: neurons,glial cells and hemocytes.3.The neurons in the brain of Apis mellifera were reclustered and the cell types of the neurons were further distinguished.4.The use of different neurotransmitters in the brains of different honeybee types was analyzed and compared.It was found that there were neurons expressing two,three or even four neurotransmitters at the same time in the brain of Apis mellifera,and compared the differences in the use of neurotransmitters between the neurons in the brains of worker and the queen.In summary,we constructed and analysis for the single-cell transcriptome atlas of worker and queen’s brain in honey bee.The specific regulatory mechanism still needs further investigation in the follow-up experiments.