The Genetic Differentiation And Differential Gene Expression Of Colletotrichum Fructicola In Different Geographic Regions

Anthracnose is a major disease that causes serious economic losses in the Chinese Camellia oleifera.Existing studies have shown that Colletotrichum.fructicola is the dominant pathogenic fungi of Camellia anthracnose in my country.The C.fructicola in different geographic regions show different pathogenicity.This study uses resequencing technology to determine the whole genome of C.fructicola from different geographic populations,and uses whole genome data to reveal C.fructicola from different geographic regions through genetic evolution relationship analysis,principal component analysis.population genetic structure analysis,and population genetic index For the genetic differentiation of C.fructicola,key differential genes were screened by comparing reference genes,and the expression of differential genes was verified by qRT-PCR.Revealing the genetic differentiation of C.fructicola in different geographic regions and their differential genes will help the targeted prevention and control of Camellia anthracnose,and it is also beneficial to the green prevention and control of Camellia anthracnose and the healthy management of the C.oleifera industry.The main research conclusions are as follows:Screening of differential genes of C.fructicola in different geographical regions:A total of 11.85Gb whole-genome resequencing data was obtained from 6 samples,and the proportions of high quality were 99.65%,99.69%,99.42%,99.65%,99.53%and 99.67%,respectively,The comparison rate of the 6 samples with the reference gene is between 91.95%and 95.24%;according to the results of SNP and InDel annotation analysis,a total of 248,135 SNP variant sites,24331 InDel variant sites,and 36026 non-synonymous mutations have been obtained SNP and 1813 coding regions frameshift InDel;for the SV detection and annotation results of the Shaoyang and Wuzhishan populations,the number of structural variations that only exist in the Shaoyang population is 37,and only 65 exist in the Wuzhishan population.There are obvious differences between Shaoyang and Wuzhishan both in the type and number of structural variations.According to the results of the CNV annotation,the number of CNVs that only exist in the Shaoyang population is 101,and there are only 216 in the Wuzhishan population.There are also obvious differences in CNV between Shaoyang and Wuzhishan.This indicates that the whole genome of C.fructicola has abundant polymorphisms between these two geographical populations.In this study,6 purine nucleosides were selected by annotating all non-synonymous mutant SNPs and coding region frameshift InDel.Acid anabolism related genes and 6 phenylalanine and β-alanine metabolism related genes.The genetic differentiation of C.fructicola in different geographic regions:genetic evolution relationship analysis,principal component analysis and population genetic structure analysis reveal the genetic differences between C.fructicola from Wuzhishan population and Shaoyang population.There are obvious genetic differences between the Wuzhishan population and the Shaoyang population,and the genetic diversity of the Wuzhishan population is significantly greater than that of the Shaoyang population;in order to further reflect the degree of genetic differentiation between the two populations,and to quantify the genetic differences between the two populations.This study analyzed the population genetic indexes of the two populations,and the results showed that the Wuzhishan population and the Shaoyang population are rich in polymorphisms.There is significant genetic differentiation between the two populations.The genetic diversity of the Wuzhishan population is both Greater than the Shaoyang population.Analysis of the differential gene expression of C.fructicola in different geographical regions:The relative expression of 6 purine nucleotide anabolism-related genes CGGC5₁1342,CGGC5₁1516,CGGC5₁3215,CGGC5₁5250,CGGC5₃653,CGGC5₅691 is low and relatively stable in the population of Shaoyang,Hunan The Wuzhishan population showed a significant up-regulation.This shows that the genes related to purine nucleotide metabolism in the Wuzhishan population in Hainan have undergone significant genetic differentiation,which makes the Wuzhishan population in Hainan more comprehensive in the regulation of purine nucleotide metabolism.Further verification showed that CGGC5₁1342,CGGC5₁1516,CGGC5₁3215,CGGC5₁5250,CGGC5₃653,and CGGC5₅691 are related to the pathogenicity of C.fructicola.The expression levels of phenylalanine and β-alanine metabolism-related genes CGGC5₁1752,CGGC5₁143,CGGC5₃992,CGGC5₆029,CGGC5₈028,and CGGC5₈827 in populations of Wuzhishan,Hainan were significantly lower than those of Shaoyang population and were relatively stable.This shows that the genes related to the metabolism of phenylalanine and β-alanine in Wuzhishan population in Hainan have obvious genetic differentiation.However,the Wuzhishan strains involved in this study did not show obvious regularities in the metabolism of phenylalanine and β-alanine.