| With the characteristics of early fruit bearing and high benefit,greenhouse grape developed rapidly in Ningxia,which significantly improved the economic benefit of local grape industry.The combination model of greenhouse Yin tent is given priority to with cultivation of grapes,but in the process of cultivation,due to weak Yin shed light,low films transmittance,plus in recent years,the frequent rain and fog weather,lead to insufficient facilities often light,grapes from bud begins in weak light stress,causing subsequent flowering difficulties,berry quality variation,It seriously affected the economic benefit of grape facility cultivation.Artificial supplementary light is an effective measure to improve the lighting conditions in the facilities.To explore different light environment facilities "Red Globe" grape bud burst and fruit quality,the effect of this research in facility cultivation "Red Globe" grape as the experimental material,using four different light quality(red and blue 2:1,blue,red,white)fill light processing,not to fill the light contrast,under different light quality processing through the grape shoots and fruit physiological indexes of bud burst and analysis of the transcriptome study,different light environment"Red Globe" grape bud burst,and the influence of fruit quality,the results are as follows:(1)Different light quality promoted the burst of grape bud and improved the quality of fruit.At the burst stage,red and blue light had the highest leaf spreading rate(81%),followed by white,blue and red light,and the control had the lowest leaf spreading rate(68%).Under the treatment of red and blue light,the contents of soluble sugar(8.88 mg·g-1),total protein(101.61 mg· mL-1)and H2O2 in buds were the highest(2.06 mmol·g-1),and the activity of CAT was the lowest(36.43 U·μg-1).At the turning stage,the contents of reducing sugar and soluble solids in the fruits treated with blue light were the highest,which were 161.83 g·L-1 and 17.13%,respectively.The contents of titratable acid in the fruits treated with blue light and red and blue light were the lowest,which were 3.35 g·L-1 and 3.20 g·L-1,respectively.The content of anthocyanin in the fruits treated with blue light was the highest,which was 0.07 mg·g-1.(2)There were differences in light response metabolic pathways between grape bud and fruit.KEGG analysis showed that differentially expressed genes were significantly enriched in the pathways of plant hormone signal transduction,endoplasmic reticulum protein processing and plant fungus interaction.Light quality regulated the burst process of grape by regulating the expression of genes related to plant hormone signal transduction.Turn color of the fruit maturity and signal transduction,carbohydrate transport and metabolism,amino acid transport and metabolism is closely related to the process,such as KEGG analysis showed that the processing main differentially expressed genes in the biosynthesis of photosynthesis,flavonoids,starch and sucrose metabolism pathway enrichment,biosynthesis and metabolism of these genes involved in turn the color of the fruit ripening,play an important role in the formation of various processing fruit quality.(3)In this study,ERF,MYB,WRKY and NAC transcription factor families were significantly enriched in grape buds and fruits,suggesting that ERF,MYB,WRKY and NAC family transcription factors play an important role in grape light response.Auxin,gibberellin,cytokinin,abscisic acid,brassinolide,WRKY transcription factors,calci-dependent protein kinase,calmodulin and NADPH oxidase form a light-responsive protein interaction network in the bud,and the interactions among these proteins co-regulate the burst of grape buds.Photosynthetic proteins,flavonoid biosynthetic proteins,starch and sucrose metabolic proteins are the main light-responsive proteins in grape fruits.The interactions between these proteins together regulate grape fruit quality.The 18 differentially expressed genes in buds and fruits were verified by qRT-PCR,and the correlation analysis between the validation results and the RNA-seq data showed that the correlation coefficient was high and the expression trend was basically the same,indicating that the RNA-seq analysis results were reliable.In conclusion,different light quality can accelerate the burst of grape bud.Red and blue light is the ideal light quality to promote the burst of grape bud.The up-regulated expression of SAUR,A-ARR,GID1,PYR/PYL and PP2C genes in plant hormone signal transduction pathway was an important reason for the difference of grape bud burst among different treatments.The color and quality of grape fruits were improved by different light supplemental quality,and blue light was the most obvious.The up-regulated expression of flavonoid genes CHS,F3H and starch and sucrose metabolism genes INV,SPS,HK and BG in grape fruits were the important reasons for the difference in fruit color and quality.This study provided a theoretical basis and data support for the light regulation technique of protected grape. |
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