Molecular Characterization,expression And Functional Analysis Of Acyl-CoA-binding Protein Gene Family In Maize

Lipid is the energy source of plant metabolism,the structural basis of cell membrane,and the signal molecule in response to stress.Lipids need to be transferred from the synthesis site to the target site to perform their function,and the Acyl-CoA binding protein(ACBP)family plays a role in transporting lipids.ACBP possess a conserved acyl-CoA-binding(ACB)domain that facilitates binding to lipid binding and trafficking in eukaryotic cells.Although the various functions of ACBP have been characterized in several plant species,has not been fully elucidated in Zea mays L.In this study,the structure,molecular characteristics,expression and function of maize ACBPs were described by homology-based cloning,phylogenetic analysis,subcellular localization,stress and regional association analysis.Genome-wide analysis identified nine ZmACBP genes in Z.mays,which could be divided into four distinct classes,ZmACBPs localized to multiple different locations,ZmACBPs exhibited temporal and spatial expression changes during abiotic and biotic stresses.Eight of the nine ZmACBP genes were also found to have significant association with agronomic traits in a panel of 500 maize inbred lines.The heterologous constitutive expression of ZmACBP1 and ZmACBP3 in Arabidopsis,enhanced the resistance of these plants to salinity and drought stress,possibly through alterations in the level of lipid metabolic and stress-responsive genes.The aim of this study was to verify the biological function and mechanism of ZmACPBs,and to provide genetic resources and theoretical basis for the creation and genetic improvement of high-quality stress-tolerant maize germplasm resources...