Stress Resistance Of SoHsfB2 Gene In Spinach

Spinach(Spinacia oleracea L.)is one or two year herbaceous plants of Amaranthaceae spinach having green leaves as the main product organ.It is cold weather and enjoys cool weather and won’t resist high temperatures.Suitable growth temperature is 18 to 22 ℃.which has adverse effects on its development and has edible value Plants may die from the threat of long-term high temperature.It is an ideal material to study the mechanism of plant rebelliousness and explore the rebellious genes.Chemical structures of abiotic stress tolerance are dependent on specific activation important genes and impose important roles of plant heat stress transcription factor(HSF)in stress response and stress tolerance.In this experiment,SP75,heat resistant spinach inbred strain,and thermosensitive inbred strain SP73 were used as research materials.Screening of proteins interacting with spinach SoHsfB2 by constructing yeast library was carried out to predict and analyze related functions.Through a transient representation system for studying and analyzing the stress resistance function of sohsfb2 Stress tolerance function of spinach SoHsfB2 gene and bioinformatics analysis of spinach WRKY and analysis of stress tolerance function of SoHsfgene transgenic Arabidopsis for analysis of heat resistant regulatory network.The research results are as follows.1.Under different abiotic stress treatment conditions,WT and SoHsfArabidopsis were tested for seed germination,root length determination and related physiological indicators,and then a comprehensive analysis of different abiotic stresses on Arabidopsis seedlings Impact.The results showed that SoHsfB2 gene can significantly improve the resistance of plants under stress conditions such as high temperature,salt and drought.2.The transient transformation system of spinach detached leaves was established by the method of Agrobacterium infection,and the system was optimized;the Agrobacterium infection activity was detected and the GUS enzyme activity of the SoHsfB2 gene was determined,indicating that the spinach SoHsfB2 gene has Significantly improve the ability of plants to resist high temperature stress.3.By constructing a yeast two-hybrid library,SoHsfB2 self-activation identification,transformation and screening of interacting proteins.Twenty-five proteins that interact with SoHsfB2 have been obtained.The candidate interacting proteins are mainly annexins involved in plant calcium ion metabolism,NAC transcription factors and ubiquitin(Ubl)that can respond to endoplasmic reticulum stress and abiotic stress.Important regulator of conjugate pathway.4.Through spinach high temperature expression profile and WRKY evolutionary tree analysis and screening,eight candidate genes that may participate in the upstream and downstream interactions of SoHsfB2 were obtained,and then the regulatory relationship was studied.