Physiological Response Of Fig To Salt Tolerance And Transcriptome Analysis Of Salt Tolerance

In this study,the fig cultivar ’Branrick’ was morphologically and physiologically changed under different concentrations of Na Cl solution to determine the growth concentration of fig tolerant to salt stress of 0.4%.In order to study the salt tolerance characteristics of different varieties of fig seedlings,0.4% Na Cl was subjected to salt stress treatment on 17 fig varieties.The experiment showed that the physiological indexes related to salt stress were significantly different among the varieties on 0,7,14,21 and 28 days.Through principal component analysis and cluster analysis,the salt tolerance characteristics of 17 fig varieties were sorted and classified into a total of There are three categories of highly salt-tolerant varieties,moderate salt-tolerant varieties and salt-sensitive varieties,including 2 highly salt-tolerant varieties,12 moderate-tolerant varieties,and 3 salt-sensitive varieties.In order to study the differentially expressed genes of fig seedlings under salt stress,two extreme differential varieties were screened out,the salt-tolerant variety ‘this morning’ and the salt-sensitive variety ‘Bo Jihong’ were analyzed by second-generation transcriptome sequencing.A total of 36.68 Gb Clean Data was obtained by sequencing.The Clean Data of each sample reached 6.56 Gb,and the Q30 base percentage was 85% or more.Transcriptome sequencing revealed 4259 differentially expressed genes,of which 2113 genes were up-regulated and 2146 genes were down-regulated.Experiments show that the differentially expressed genes are closely related to signal transduction mechanisms,post-translational modifications,protein renewal,transport and catabolism,carbohydrate transport and metabolism.Salt stress is a complex metabolic process.This experiment combined with physiological response research and analysis,analyzed the gene expression of salt stress transcriptome of different salt-tolerant varieties of figs,and had a deeper understanding of the molecular level regulation of figs And changes,and at the same time constructed a library of fig transcriptomes under salt stress conditions,which provides basic research results for further study of the molecular mechanism of fig salt tolerance.