| Zhuzishen(Panax japonicus var.major)is the dried rhizome of a medicinal plant of the Araliaceae genus.It has the effects of "anti-arteriosclerosis,improving blood lipids,blood pressure and blood sugar".At present,the research on Zhuzishen is mainly related to chemical composition,and its genome data has not been reported yet.Based on the second-generation and third-generation sequencing technologies,this paper obtained the organelles and nuclear genome data of Zhuzishen,and combined with transcriptome data to annotate and compare the nuclear genome.The analysis results revealed the characteristics of Zhuzishen’s organelles and nuclear genome.In addition,also analyzed the horizontal transfer of genes.(1)The assembly results showed that the nuclear genome size was 2.26 Gb with AT accounts for 64.27%,and all contigs were greater than 1 Kb.1614 genes were tested in the quality assessment of genome assembly,and the BUSCO value reached above 80%.In this study,a total of 195258 DNA transposons were identified,with a total length of 0.02 Gb.(2)The total length of the chloroplast genome is 156159 bp with AT accounts for61.93%,which is a standard tetrad structure.The mitochondrial genome of Zhuzishen is composed of three circular DNA molecules with lengths of 288009 bp,173383 bp and 94358 bp with AT accounts for 55.32%/54.40%/55.55%.(3)Repetitive sequence analysis identified 50 repeats in chloroplast genome,and150 repeats in three mitochondrial genomes.(4)A total of 43 simple repetitive sequences(SSR)were identified in the chloroplast genome and mitochondrial genomes.(5)It is predicted that there are 144 RNA editing sites in the chloroplast genome,and 216 RNA editing sites in the mitochondrial genomes.(6)The results showed that the Panax japonicus,Panax japonicus var.major and Panax japonicus var.bipinnatifidus has the closest relationship.(7)In the 23 identified fragments,17 of them have no function in mitochondria,and the remaining 6 were t RNAs.Mt1,Mt2 and Mt3 have 7/5/11 fragments respectively.(8)Gene codon preference may be mainly affected by low GC content and AT terminal codon preference. |
