| Alfalfa is a high-quality main forage,and its production performance directly determines the supply of forage.In order to clarify the factors of the formation of heterosis between F1 generations of alfalfa from different parents,as well as the analysis of the combining ability and production performance of alfalfa hybrid combinations,the application of alfalfa hybrid breeding and breeding was carried out.This thesis uses the hybrid combination materials selected in the previous stage.One is the female parent of alfalfa cytoplasmic male sterile line MS-GN-1N and 4excellent alfalfa materials as the male parent and the corresponding F1 generation.The agronomic traits,phenotypic traits,and quality traits of individual plants in F1 generation were analyzed to evaluate their combining ability,heterosis level and its forming factors.The second is to compare the F1 generation obtained from the cross with the local superior variety,and analyze the yield performance of the hybrid combination and the local superior variety.The test results are as follows:1.From the perspective of agronomic characteristics,compared with the local variety Gongnong No.1,the F1 generation hay yield and plant height obtained from the cross of the tested alfalfa parents were higher than those of the parents and the control,but the dry grass yields and plant heights between the various combinations and varieties were higher.The difference in freshness ratio and stem-leaf ratio is not very significant.From the perspective of phenotypic traits,the number of F1 generations of alfalfa is higher than that of parents,but the changes in leaf area,internode length and stem thickness are not very significant.From the results of quality trait analysis,the crude protein content of the tested alfalfa varieties reached the highest in the first crop,followed by the second crop,and the lowest in the third crop.The trend of the crude fiber content was the opposite.From the annual average change,the alfalfa The crude protein content of the F1 generation of alfalfa was higher than that of the parents,and the crude fiber content of the parents was higher than that of the F1 generation.However,the changes in crude fat,crude ash and moisture were not very large.2.The analysis of variance for factors such as agronomic traits,phenotypic traits,and quality traits shows that six factors including hay yield,node number,plant height,branch number,crude fiber,and crude protein are significant among parents and hybrid combinations.Variety.Combining ability analysis shows that WL-323ML(F1)has the highest special combining ability effect value of 22.68,followed by multi-leaf alfalfa(F1)with 22.11,and Pyramid(F1)has a special combining ability effect value of-0.77,indicating that WL-323ML(F1)has the best overall performance.The yield correlation analysis showed that the yield was extremely significantly positively correlated with the number of branches,stem thickness,and node number(P<0.01),and significantly positively correlated with leaf area(P<0.05).Cluster analysis comprehensively compares the above 6 factors,and the results show that the F1 generation factors perform better.3.In the taste comparison test,the overall performance of multi-leaf alfalfa(F1)and WL-323ML(F1)are outstanding,and they are suitable for large-scale planting in western Jilin.At the same time,the results of cluster analysis also showed that the yield and production potential of alfalfa hybrids were relatively high,while the yield and production potential of sterile lines was relatively low. |