Transcriptome Analysis Of Oilseed Rape In Response To Potassium Deficiency Stress And Identification Of Diffentially Express Genes In The CPA Family

Reasonable application of potassium fertilizer can significantly increase the yield and quality of oilseed rape,while potassium fertilizer can significantly increase the photosynthetic capacity of crops,which is an important reason for potash fe rtilizer to increase crop yield.A lot of work has been done on potassium and crop photosynthesis.The redistribution of potassium ions between chloroplast and cytoplasm is closely related to photosynthesis,but its mechanism of action is not clear.The monovalent cations and proton reverse transport proteins(The monovalent cations and proton reverse transport proteins,CPA)mediate the redistribution of potassium ions between cells and sub-cells.Among them,KEA1,KEA2,and KEA3 are located in the chloroplast.It has a greater impact on leaf photosynthesis.Therefore,exploring the effect of potassium nutrition on the expression of leaf photosynthesis-related genes and CPA family genes will help to understand the biological mechanism of potassium regulating leaf photosynthesis of oilseed rape.This study used field and hydroponic experiments,using transcriptomics and bioinformatics methods,to clarify the differentially express genes related to photosynthesis and the differentially express genes of the CPA family in response to potassium deficiency stress,in order to understand the role of potassium in regulating leaf photosynthesis of oilseed rape,and to provide the key support for biological mechanism.The main results are as follows:(1)Potassium deficiency stress is not conducive to the normal growth of oilseed rape leaves.The potassium content and net photosynthetic rate of each part leaves were reduced.The potassium deficiency symptoms of the lower leaves were more obvious when faced with potassium deficiency.On the one hand,the lower leaves which were treated with different potassium,the number of differentially expressed genes(DEGs)of which was more than that of the upper leaves.On the other hand,the number of DEGs between the upper and lower leaves of oilseed rape under the potassium deficiency treatment was more than that of the sufficient potassium treatment.Preliminary screening of 101 DEGs related to photosynthesis in response to potassium deficiency stress,including photosystem II oxygen evolution complex protein Psb P gene,ferredoxin coenzyme Pet H gene and so on.(2)The basic information of 106 oilseed rape CPA family genes was compared and screened,including 73 CHX family genes,18 NHX family genes and 15 KEA family genes.The gene structure of the CPA family of oilseed rape is relatively conservative,and it is unevenly distributed on 41 chromosomes of oilseed rape.A total of 12 DEGs in the CPA family of oilseed rape in response to potassium deficiency stress were screened,and they were differentially expressed in different potassium treatments at the bud and shoot stage.(3)There was no difference in the expression of KEA1 in Arabidopsis leaves under short-term potassium deficiency,while the expressions of KEA2 and KEA3 were downregulated and up-regulated respectively;under long-term potassium-deficiency stress,there was no difference in the expression of KEA1,the expression of KEA2 was down-regulated,and the expression of KEA3 was up-regulated;There was no difference in the expression of KEA1 under the stress of long-term potassium deficiency and the potassium supply was restored.The expression of KEA2 was first down-regulated and then up-regulated,and the expression of KEA3 was up-regulated and then down-regulated.