Molecular Evaluation Of Vitality,Survival Rate And Comparison Of Field Planted Seedlings To Soil Burial For Overwintering Of Dormant Kyoho Grape Seedlings

Vitality and survival rate of grape seedlings are crucial factors affecting quality of vineyards.Winter survival of seedlings is one of important stage in a plant life and survival rate of the seedlings is elucidating number of living seedlings in all.There is no comprehensive study describing accurate evaluation of dormant grapevine seedlings’vitality and survival rate.The purpose of this study was to explore the possibility of using molecular information to evaluate vitality and survival rate of dormant seedlings before transplanting and to compare soil burial to field planted grape seedlings for overwintering.In addition,to compare winter survival resistance of the seedlings produced in different provinces.After bare roots treatment,1-5 day expression levels of six HKGs in four buds of tetraploid Kyoho grape(Vitis labruscana:V.labrusca×V.vinifera)seedlings were detected by(Sq.)RT-PCR and qRT-PCR for calibration of the molecular method.The seedlings obtained from Hebei,Jiangsu and Shandong provinces were selected to compare soil burial to field planted for overwintering.The results revealed that HKGs expression indicates vitality and survival of plant,higher expression was strongly linked to higher vitality and survival rate,lower expression was associated with lower vitality,and lowest expression was significantly associated with lowest vitality and survival rate.Moreover,DNA and RNA quality can superficially determine seedling qualities.Finally,the survival rate of the seedlings produced in Juxian-Shandong,Laixi-Shandong,Huailai-Hebei,Suizhong-Liaoning,Changli-Hebei,Guanxian-Shandong,and Zhangjiagang-Jiangsu was 100.00%,100.00%,100.00%,100.00%,100.00%,87.77%,and 93.33%,respectively.The survival rate of the seedlings buried in soil was higher compare to the seedlings planted in the field for overwintering.In addition,the seedlings obtained from all mentioned provinces placed in soil burial exposed higher survival rate while the seedlings planted in pots showed different reactions of survival against cold winter.Furthermore,the survival rate of the seedlings planted in field for overwintering obtained from Hebei,Jiangsu and Shandong provinces were 87.77%,81.11%and 77.77%respectively.In conclusion,molecular technique is potential approach for promoting gene information to estimate vitality and survival rate of dormant grape seedlings and might contribute to viticulturists’efforts.Remarkably,the winter seasonal effects substantially decreased seedlings survival in Nanjing province due to cold stress during dormancy.Accordingly,Kyoho grape seedlings needs soil burial to successfully survive during winter.