Bioinformatics Analysis And Gene Expression Analysis Of MADS-box And Class Ⅲ PRX Gene Family In Setaria Italica Under Drought

The MADS-box gene family is an important family of transcription factors that play a crucial role in plant reproductive development and stress resistance.Class III peroxidases are a family of peroxidases unique to plants and are involved in growth hormone metabolism,lignin formation,metabolism of reactive oxygen species and resistance to abiotic stresses.Foxtail millet,as a C4 plant,is a model plant for stress resistance research in the grass family.To investigate the molecular mechanisms of the MADS-box family(SitMADSs)and class Ⅲ PRX gene family(Sit PRXs)in response to drought stress,MADS-box gene family and class Ⅲ PRX gene family were identified and the expression patterns under drought stress were analyzed of the using Yugu 1 as the material.The main results showed that:1.A total of 70 SitMADS gene members were identified.The results of the colinearity analysis showed that SitMADS genes have tandemly duplication and segemental duplication events and were more homologous to the MADS-box gene family in rice.2.The results of real-time quantitative PCR analysis showed that the ten SitMADS genes examined were tissue expression specific in roots.SitMADS23,SitMADS42,SitMADS51,SitMADS52,SitMADS58 and SitMADS64 were highly expressed in PEG and drought stress.Meanwhile,SitMADS51,SitMADS63 and SitMADS64 were responsive to exogenous ABA hormone signals.The full-length coding sequences of SitMADS42 and SitMADS64 were cloned,and the amplified sequences were 1347 bp and 750 bp,respectively.3.A total of 132 Sit PRX gene family members were identified and classified into five subfamilies by phylogenetic analysis in Setaria italica,Arabidopsis thaliana and Oryza sativa.Gene duplication analysis revealed that tandemly duplication events play an important role in Sit PRX gene amplification.Interspecies colinearity analysis of foxtail millet with Arabidopsis thaliana,Oryza sativa and Zea mays revealed that most Sit PRXs were formed after dicotyledonous and monocotyledonous plants diverged.4.Transcriptome analysis revealed that members of the Sit PRX gene family were differentially expressed in seedlings,roots,stems,leaves and panicles in foxtail millet.Real-time quantitive PCR analysis showed that Sit PRX12,Sit PRX41,Sit PRX81,Sit PRX110 and Sit PRX126 were induced to be expressed by PEG and ABA.The full-length coding sequence of Sit PRX126 was cloned,and the amplified sequence length was 753 bp.