| Objective: This study intends to explore the factors that affect the prevalence of T2 DM and the association between VCAM1 gene polymorphism and T2 DM and physiological and biochemical indicators through case-control studies,which will provide a reference for the research on the prevention,morbidity and treatment of T2 DM.Methods: T Taking the medical examination population of the First Affiliated Hospital of Guangxi Medical University as the research object,a case-control study method was used,and after screening according to the inclusion criteria and exclusion criteria,a total of 1945 research subjects were included,including 922 cases in the case group and 1023 cases in the control group.VCAM1 genotyping was obtained by questionnaire survey and physical examination to obtain the basic information of the research object,blood test biochemical indicators and DNA extraction.Use Epidata 3.1 software to double-enter data to establish a database.Havin’s equilibrium test,allele and genotype analysis are completed by Plink 1.07 software,linkage disequilibrium analysis is completed by SHEsis online platform;logistics regression model is used for gene-environment interaction Function analysis,the rest of the statistical analysis was performed using SPSS 23.0software.Results:(1)39.26% males and 60.74% females in the case group,38.22%males and 61.78% males in the control group,and there was no significant difference in gender between the two groups(P=0.638).The smoking rate in the case group was 10.20%,and the smoking rate in the control group was 7.53%.The case group had a higher smoking rate than the control group(P=0.038).In the case group,the number of central obesity accounted for 70.17 % of the total number,higher than 51.61% of the control group(P<0.001);the case group has24.19% of primary school education and below,36.01% of junior high school education,27.98% of high school or technical secondary school education,11.82%of college education and above,and the control group of primary school education and below 18.75% of education,34.41% of junior high school education,33.33%of high school or technical secondary school education,13.69% of junior college education and above,there is a significant difference in the distribution of education between the two groups(P<0.001).37.74% of the patients in the case group have normal BMI,44.58% overweight,17.68% obese,50.34% normal BMI in the control group,40.57% overweight,9.09% obese,the case group had a higher overweight rate and obesity rate than the control group(P<0.001).Body weight,waist circumference,hip circumference,SBP,DBP,FINS,PBG,FBG,2h PG,Hb A1 c,CHOL,TG levels were higher in case group than the control group(P<0.05),HDL levels of the case group were lower than the control group(P<0.05).(2)The risk of T2 DM among smokers is 0.496 times higher than that of nonsmokers(OR=1.496,P=0.035).The risk of T2 DM in central obesity is 1.720 times that of normal WHR(OR=1.720,P<0.001).The risk of T2 DM in obese is1.729 times that of normal BMI(OR=1.729,P=0.001).SBP and TG levels increase,the risk of T2 DM increases(P<0.001),HDL levels increase,the risk of T2 DM decreases(OR= 0.546,P=0.001).(3)The rs1041163,rs1409419,and rs3783621 loci all conformed to the Havin law of genetic balance(P>0.05).It was found in the stratified analysis that the T<C mutation at the rs1409419 locus in the female population reduced the risk of T2DM(OR=0.827,P=0.025),the individuals with CC\TC genes in the dominant model have a lower risk of T2 DM compared with TT genotypes(OR=0.702,P=0.008).In the codominant model,individuals with TT genotypes have T2 DM risk is 0.832 times that of individuals with CC genotype(OR=0.832,P=0.031).(4)Gene-environment interaction analysis found that there is a multiplicative interaction between rs1409419 and WHR and smoking.The central obese person with TT gene is 1.568 times higher than the normal WHR with TT genotype(OR=2.568,P<0.001).The central obese person with CC\TC gene has 1.962 times the risk of T2 DM compared with the normal WHR with AA genotype(OR=1.962,P<0.001).Compared with individuals with non-smoking TT genotype,individuals who smoked CC\TC genotypes had a 0.485-fold higher risk of developing T2DM(OR=1.485,P=0.047).There is a multiplicative interaction between the rs3783621 locus and WHR.Central obesity with AA gene is more normal than AA genotype WHR the risk of T2 DM was 0.894 times higher(P<0.001),the central obesity of CC\AC gene was 0.915 times higher(OR=1.915,P=0.001).(5)In the male population,under the dominant genetic model,the SBP level of the TT genotype population at rs1409419 was higher than that of the CC\TC genotype population(P<0.05).(6)In the female population,the FBG and TG levels of the TT genotype rs1041163 were higher than those of the CC\TC genotype(P<0.05).The TT genotype rs1409419 was higher than the CC\TC genotype Hb A1 c,FBG and 2h PG levels(P<0.05).The LDL level of rs3783621 CC genotype population was higher than that of AA\CA genotype population(P<0.05).Conclusions:(1)Obesity,central obesity,elevated systolic blood pressure,and elevated TG are risk factors for T2 DM and elevated HDL levels is protective factors for T2 DM.(2)There is a multiplicative interaction between rs1409419 and WHR and smoking,which may be related to the increased risk of T2 DM.In the female population,rs1409419 may be related to the prevalence of T2 DM and Hb A1 c,FBG,2h PG levels.In men Rs1409419 may be related to SBP in the population.(3)There is a multiplicative interaction between rs3783621 and WHR,which may be related to the increased risk of T2 DM.In female population,rs3783621 may be related to LDL levels.(4)The rs1041163 locus may be related to TG and FBG levels in the female population. |
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