Based On Histone Deacetylase To Explore The Possible Mechanism Of WuTou-decoction In Treating Rheumatoid Arthritis Synovial Angiogenesis

Purpose To investigate the effect of Wutou Decoction on the treatment of rheumatoid arthritis,and to explore its effects on the phenotypes of fibroblast-like synovial,including cell proliferation,apoptosis,migration,invasion,and the expression of histone deacetylase.Methods The fibroblast-like synoviocytes were treated with the extract of the main component of Wutou decoction,the proliferation activity of FLS was detected by MTT,the apoptosis of FLS was detected by flow cytometry,the migration ability of RA-FLS was measured by scratch test,and the RA was detected by Transwell detect FLS invasive,tube formation experiments to detect FLS’s ability to induce angiogenesis.Then,the HDAC expression of FLS was detected by PCR,and further verified by immunofluorescence and Western blot.Wistar rats were injected with bovine type II collagen to construct a CIA model.The 30 rats successfully modeled were divided into a model group,a low-dose Wutou decoction group(WTD-L),and a high-dose Wutou decoction group(WTD-H)Three groups of 10 animals each.The Wutou decoction was administered orally for 28 days,and the normal control group and the CIA group were orally administered with normal saline.All rats were sacrificed at the end of the experiment and knee synovial membranes were collected.Immunohistochemical detection of synovial angiogenesis,immunofluorescence detection of HDAC7 expression in synovial cells.Results Wutou decoction can inhibit the proliferation,migration,invasion,and ability to induce angiogenesis of fibroblast-like synovial cells,and down-regulate the expression of fibroblast-like synovial cells HDAC7.In-vivo experiments further verified that Wutou decoction can relieve joint swelling and pain in CIA rats,improve joint function,inhibit synovial hyperplasia,inhibit synovial angiogenesis,and down-regulate HDAC7 expression.Conclusion Wutou decoction can inhibit the proliferation,migration,invasion of fibroblast-like synovial cells,and induce the formation of vasculum to improve joint swelling.Its effect on synovial pannus hyperplasia may be related to the inhibition of HDAC7 expression in fibroblast-like synoviocytes.