| Objective:To compared the correlation and consistency between morning urine and24-hour urine indicators of urinary calculi patients and non-calculus patients after creatinine correction,to evaluated whether morning urine urine composition analysis can replace 24-hour urine composition analysis for clinical metabolism assessment.Trials and Methods99 patients with urinary calculi who were hospitalized in our hospital from January 2020 to January 2021 were selected as the stone group,and 20 Non-calculus patients with physical examination were selected as the Non-calculi group during the same period.All participants in the study were retained 24-hour urine and morning urine,use automatic biochemical analyzer to automatically detect calcium,potassium,sodium,magnesium,phosphorus,creatinine,uric acid and other components in single urine and 24-hour urine,using Thermo Fisher Diane ICS-600 Ion chromatograp H detects phosphoric acid,oxalic acid,citric acid and other components in single urine and 24-hour urine.Pearson correlation analysis was used to judge the correlation between morning urine and 24-hour urine in the stone group and the Non-calculi group;intraclass correlation efficient(ICC)was used to evaluate the consistency of morning urine and 24-hour urine in the stone group and the Non-calculi group;the receiver operating characteristic(ROC)curve was used to evaluate the diagnostic value of morning urine oxalate and citric acid levels for hyperoxaluria and hypocitrateuria.Result(1)The results of this study showed that there was no statistically significant difference in the incidence of hypernatremia,hypomagnesuria,hyperuricuria,and hyperphosphouria between the stone group and the non-calculus group in age,gender,and 24-hour urine metabolism analysis(P > 0.05).The incidence of low urine output,high oxaluria,low citrateuria,and hypercalciuria in BMI and 24-hour urine metabolism analysis in the stone group was significantly higher than that in the non-calculi group(P < 0.05);(2)There was a statistically significant difference between morning urine and24-hour urine oxalate/creatinine,citrate/creatinine,phosphoric acid/creatinine,calcium/creatinine,magnesium/creatinine,sodium/creatinine,chloride/creatinine levels in the stone group(P < 0.05),potassium/creatinine There was no statistically significant difference in creatinine,phosphorus/creatinine,and uric acid/creatinine levels(P > 0.05);there were statistically significant differences in morning urine and24-hour urine citrate/creatinine,phosphoric acid/creatinine,and magnesium/creatinine levels in the non-calculi group(P < 0.05),there was no significant difference in the levels of oxalic acid/creatinine,calcium/creatinine,potassium/creatinine,sodium/creatinine,chloride/creatinine,phosphorus/creatinine,uric acid/creatinine(P > 0.05).(3)The Pearson method correlation analysis results showed that there was a positive correlation between morning urine and 24-hour urine components in the stone group with Oxalic acid/creatinine,citrate/creatinine,phosphoric acid/creatinine,calcium/creatinine,magnesium/creatinine,potassium/creatinine,sodium/creatinine,chloride/creatinine,phosphorus/ Creatinine,uric acid/creatinine(r=0.511、0.476、0.313、0.339、0.321、0.365、0.314、0.370、0.386、0.409,P<0.05).There was a positive correlation between morning urine and 24-hour urine components in the non-calculi group with Oxalic acid/creatinine,citrate/creatinine,phosphoric acid/creatinine,calcium/creatinine,magnesium/creatinine,potassium/creatinine,sodium/creatinine,chloride/creatinine,phosphorus/ Creatinine,uric acid/creatinine(r=0.707,0.596,0.541,0.669,0.565,0.581,0.474,0.551,0.687,0.671,P<0.05);(4)After intraclass correlation efficient(ICC)analysis,morning urine in the stone group compared with 24-hour urine,the ICC values of oxalate/creatinine,citrate/creatinine,phosphoric cid/creatinine,calcium/creatinine,magnesium/creatinine,potassium/creatinine,sodium/creatinine,chlorine/creatinine,p Hosp Horus/creatinine,and uric acid/creatinine were 0.583,0.412,0.199,0.331,0.220,0.401,0.170,0.189,0.407,0.429,respectively(P < 0.05);Non-calculi group morning urine compared with 24-hour urine,the ICC values of oxalate/creatinine,citrate/creatinine,p Hosp Horic acid/creatinine,calcium/creatinine,magnesium/creatinine,potassium/creatinine,sodium/creatinine,chlorine/creatinine,phosphorus/creatinine,uric acid/creatinine were 0.752,0.615,0.478,0.604,0.412,0.558,0.612,0.523,0.560,0.424,respectively(P < 0.05).(5)The ROC results showed that the area under the curve(AUC)that the oxalic acid level in morning urine predicted for hyperoxaluria was 0.811(95%CI: 0.695 ～0.926),and the cut-off value was 15.89 mg/L.At this time,the corresponding sensitivity was 80%,and the specificity was 76.2%.The AUC for predicting low citrate levels in morning urine was 0.794(95%CI: 0.682 ～ 0.907),the cut-off value was 154.08 mg/L,the corresponding sensitivity was 75%,and the specificity was78.3%.Conclusion(1)There is a correlation between morning urine and 24 h urine in patients with urinary calculi and non-calculi after correction for creatinine.(2)Oxalic acid,citric acid,potassium,phosphorus,and uric acid in the morning urine and 24-hour urine of patients with urinary calculi are consistent after creatinine correction.(3)Oxalic acid,citric acid,phosphoric acid,calcium,,potassium,sodium,phosphorus,and uric acid in morning urine and 24-hour urine of non-calculi patients are consistent after creatinine correction.Reliable data can be obtained by using morning urine for metabolic assessment during a large number of population examinations.(4)Morning urine oxalate and citric acid levels have diagnostic value for hyperoxaluria and hypocitrateuria,and can be used as a simple method for clinical prediction of hyperoxaluria and hypocitrateuria. |
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