Effect Of Sympathetic Nerve Excitability Regulation On Early Inflammatory Factors And Lung Injury In Rats With Traumatic Brain Injury

Objective: By regulating the early sympathetic excitability of rats with traumatic brain injury,to explore the changes of serum adrenaline,IL-1β,IL-6,TNF-α after brain injury and their relationship with lung injury.Methods: 90 clean and healthy SD rats were randomly divided into 9 groups: blank control group(A group),traumatic brain injury 2h group(B group),traumatic brain injury 4h group(C group),traumatic brain injury 6h group(D group),sham operation group(E group),traumatic brain injury + cervical sympathetic trunk removal group(F group),traumatic brain injury + Normal saline group(G group),traumatic brain injury + hydrochloric acid Esmolol group(H group),sham operation +Normal saline group(K group).The Feeley free-falling brain injury strike device was used to prepare traumatic brain injury models.After the traumatic brain injury model was established in groups B,C,and D,they were sacrificed 2,4,and 6 hours later and serum samples were collected;group E was exposed Rat skull without impact;F group was treated with transection of the cervical sympathetic trunk(TCST)after the traumatic brain injury model was established.After 2 hours,the rats were sacrificed and blood and specimens were taken;G group After the traumatic brain injury model was established,0.9 normal saline 1ml/h was pumped for 6hours,and the blood was sacrificed at 3,6,and 9 hours.After the traumatic brain injury model was established,the H group was pumped with moxa Smolol injection was 15mg/kg/h for 6h,and the blood was taken after 3,6,and 9h.The K group was based on the sham operation group,and 0.9normal saline 1ml/h was pumped for 6 hours.,And put to death at 3,6,and9 h and take blood.The Elisa method was used to detect the levels of serum adrenaline,IL-1β,IL-6,and TNF-α in rats,and paraffin sections of lung tissues were made and HE stained.Pathological changes and lung injury pathological scores were under an optical microscope.Results:1.Changes of serum adrenaline,IL-1β,IL-6 and TNF-α levels in rats with traumatic brain injury.1.1 Compared with group B,group C,and group D,serum adrenaline,IL-1β,IL-6,and TNF-α levels increased(p<0.01);group C,group D,and group B compared with group A,serum adrenaline The levels of,IL-1β,IL-6,and TNF-α were increased(p<0.01);the serum adrenaline,IL-1β,and IL-6 levels of group D were higher than those of group C(p<0.01).1.2 The serum levels of IL-1β,IL-6 and TNF-α in group B were higher than those in group E and F(p<0.01).1.3 Comparison of rats in the same experimental group in G,H,and K groups at different time points: Changes in serum IL-1β levels:Serum IL-1β levels in group K were reduced at 3h compared with 9h in the same group(P <0.05).Serum IL-6 level changes: Serum IL-6 level increased at 9h in G group compared with 3h in the same group(P<0.05);serum IL-6 level increased at 9h in H group compared with 3h in the same group(P<0.05).Serum TNF-α level changes: The serum TNF-α level of G group was increased at 9h compared with the same group at 3h(P<0.05);the serum TNF-α level of H group was increased at 9h compared with the same group at 3h(P<0.05).1.4 Comparison of rats in different experimental groups in G,H,and K groups at the same time point: Changes in serum IL-1β levels: Serum IL-1β levels increased in G group 3h compared with H group and K group3h(p<0.05);G group Serum IL-1β levels increased at 6h compared with group H and group K at 6h(p<0.05).Serum IL-1β levels increased at 9h in group G compared with group H and 9h in group K(p<0.05).Serum IL-6 level changes: Serum IL-6 levels increased at 3h in group G compared with group H and group K at 3h(p<0.05);serum IL-6 levels increased at6 h in group G compared with group H and group K at 6h(p<0.05);The level of serum IL-6 in group G was increased at 9h compared with group H and group K at 9h(P<0.05).Serum TNF-α level changes: Serum TNF-α level increased at 3h in group G compared with group H and group K at3h(p<0.05);serum TNF-α level increased at 6h in group G compared with group H and group K at 6h(p<0.05);The level of serum TNF-α increased at 9h in group G compared with group H and group K at 9h(p<0.05).2.Pathological changes of lung tissue,pathological score of lung injury(IQA):2.1 Compared with group A,lung injury IQA of group B,group C,and group D increased(p<0.01);compared with group B,group C and group D had more severe lung injury(p<0.01);group D had more lung injury than group C The damage was severe(p<0.01).2.2 Compared with group B,group E and group F,lung injury IQA decreased(p<0.01).2.3 Comparison of rats in the same experimental group in G,H,and K groups at different time points: IQA increased at 9h in G group and6 h in G group(p<0.05);IQA decreased at 3h in G group and 6h and 9h in G group(p <0.05);Compared with group H at 9h and 6h,IQA was higher than group H at 3h(p<0.05).2.4 Comparison of rats in different experimental groups in G,H,and K groups at the same time point: IQA increased in group G at 3h compared with group H and group K at 3h(p<0.05);group G at 6h compared with group H and group K at 6h,IQA Increased(p<0.05);IQA decreased(p<0.05)at 9h in group H and K compared with 9h in group G.Conclusions:1.There is high sympathetic nerve excitement in the early stage of traumatic brain injury in rats,and peripheral serum IL-1β,IL-6,TNF-αlevels and lung tissue damage gradually increase with the injury time.2.Blocking sympathetic nerve excitement reduces the levels of peripheral serum IL-1β,IL-6,and TNF-α in rats,and reduces lung tissue damage.3.Reducing sympathetic excitability can reduce the levels of peripheral serum IL-1β,IL-6,and TNF-α,and reduce lung tissue damage.