Construction Of CircRNA-related CircRNA-miRNA-mRNA Regulatory Network In Osteosarcoma Based On High-throughput Sequencing And Analysis Of Relevant Mechanisms

Aims: the purpose of this study is to construct a competitive endogenousRNA(ceRNA)network based on the high-throughput sequencing of circRNA,miRNA and mRNA related to osteosarcoma.using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses,we further clarified mechanism of circRNA in osteosarcoma.methods: The sequencing data of circRNAs,microRNAs(miRNAs),and mRNA were acquired from Gene Expression Omnibus(GEO)datasets.By analyzing the dataset consisting of control groups and tumor groups,differentially expressed circRNAs,miRNAs and mRNAs were collected.and then the intersection of circRNAs,miRNAs,mRNAs was screened.According to these intersectionalRNAs,we constructed an integrally circRNA-miRNA-mRNA network.Finally,using Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)analyses,we further explore the regulation mechanism of circRNA.results: we obtained 45 differentially expressed circRNAs(DEcircRNAs),196 differentially expressed miRNA(DEmiRNA)and 1669 differentially expressed mRNAs(DEmRNAs),which contained 20 co-expressed circRNA,15 co-expressed circRNA and516 co-expressed mRNAs.Finally,based on co-expressed circRNA,miRNAs and mRNAs,an integrally circRNA-miRNA-mRNA network was constructed.GO analysis which contained that cell biological process(BP),cellular localization(CC)and molecular function(MF),mainly uncovered that gens were closely associated with cell migration,cell morphological regulation and function of growth factors and so on.KEGG analysis showed that co-expressed mRNAs were involved in tumor necrosis factor(TNF)signal pathway,PI3K-Akt signal pathway,MAPK signal pathway,Rap1 signal pathway,Ras signal pathway,c GMP-PKG signal pathway.Conclusion: this study provides a new insight for circRNA to mediate the occurrence and development of osteosarcoma through circRNA-miRNA-mRNA regulatory network.