The Relationship Between Rs2073618 And Rs3102735 Polymorphisms Of OPG Gene And Bone Metabolism In Postmenopausal T2DM Women

Object:By measuring the bone mineral density(Bone mineral density,BMD)and the indexes of bone metabolism,glucose metabolism and lipid metabolism in postmenopausal women with type 2 diabetes mellitus(T2DM)in Shihezi,Xinjiang;and measuring their osteoprotegerin(osteoprotegerin,OPG)gene polymorphisms and mutations at rs2073618 and rs3102735 sites;further analysis of the relationship between the two provides a basis for the explanation of the mechanism of osteoporosis and disease prevention in this population.Method:1.Collect 200 postmenopausal women in Shihezi area and divide them into four groups according to medical history,oral glucose tolerance test(OGTT)and bone density:normal glucose tolerance with normal bone mass group(group A),normal glucose tolerance group with abnormal bone mass(group B),T2 DM with Normal bone mass group(group C),T2 DM with abnormal bone mass group(group D).2.Use automatic biochemical analyzer to measure bone metabolism index,glucose metabolism index,lipid metabolism index;and use high pressure liquid method to measure Glycated hemoglobin(Hb A1c).3.Use dual-energy X-ray bone densitometer to detect its BMD.4.Collect the peripheral venous blood of the research subjects,centrifuge them,and extract DNA from them.Use time-of-flight mass spectrometry to determine the polymorphisms of OPG gene rs2073618 and rs3102735,and perform SNP detection to explore the polymorphism of gene frequency.5.Use SPSS22.0 statistical software for data processing.Variables are expressed in the form of(（？） + s).The measurement data between the two groups such as baseline data are all measured by t test,and multiple groups are analyzed by variance analysis;P<0.05 is considered statistically different.Result:1.There are statistical differences in age and menopausal years between the four groups,and the baseline is not uniform.2.After covariance analysis,compared with group A,the fasting blood glucose(FPG)and Hb Alc levels of group C and D increased;Hb A1 c level of group C increased;group B and group D L1-4 and the BMD level of the femoral neck decreased(P<0.05).3.OPG gene locus accords with Hardy-Weinberg genetic balance law,(P>0.05).4.There was no statistical difference in the frequency distribution of genotypes among the four groups,(P>0.05).5.Comparison of the basic information of different genotypes of wild type and mutant type of OPG gene locus and comparison of glucose,lipid and bone metabolism indicators:1)rs2073618 locus:In group D,the mutant type(GG/GC type)FPG and Hb A1 c are lower than wild type(CC type),mutant type(GG/GC type)TG,HDL-C are higher than wild type(CC type),mutant type(GG/GC type)Ca,BMD(L1-4)Higher than the wild type(CC type),(P>0.05).2)rs3102735 locus:In group A,Hb A1 c of the mutant(CC/TC type)is higher than that of the wild type(TT type),and the TG of the mutant(CC/TC type)is higher than that of the wild type(TT type).Type(CC/TC type)HDL-C is lower than wild type(TT type),(P<0.05);in group C,mutant type(CC/TC type)has lower Ca than wild type(TT type),(P<0.05);In group D,the mutant type(CC/TC type)has higher age and menopausal years than wild type(TT type);the baseline is uneven,and the age and menopausal years are corrected by covariance analysis.It shows:mutant type(CC/TC type)Ca is lower than wild type(TT type),(P<0.05).6.Multiple linear regression analysis:In group D,the decrease of BMI,TG and the increase of menopausal years are risk factors for the decrease of BMD(L1-4);the decrease of HDL-C and the increase of menopausal years are BMD(femur Neck)risk factors for decline.Conclusion:1.The genetic polymorphisms of OPG gene rs2073618 and rs3102735 have nothing to do with the bone metabolism of postmenopausal women with T2 DM in Shihezi area,but their mutations are involved in the occurrence of abnormal bone metabolism and osteoporosis.2.The mutation of OPG gene rs2073618 locus is related to the abnormal glucose and lipid metabolism of this population.