Identification Of Biomarkers For Minimal Change Disease Based On LMD-DIA Technology

Background: Minimal change disease(MCD)is a common glomerular disease.Timely diagnosis and intervention are particularly important for prognosis.However,the specific pathogenic mechanism and diagnostic markers are still unclear.Therefore,we explore the potential biomarkers and pathogenic mechanisms of MCD at the protein molecular level of renal tissue substructure.Methods: The kidney tissues of 5 patients with MCD were selected as the experimental group,and 5 normal kidney tissues were used as the control group.The kidney tissue was processed and made into 6um sections,and the glomerulus,renal interstitium and renal tubular tissue were obtained by laser microdissection(LMD),and the data-independent acquisition(DIA)technology was used for protein quantitative and qualitative analysis.The difference multiple>2 and Q value<0.05 were used as the criterion of significant difference to screen different proteins,and GO enrichment analysis,KEGG pathway analysis and PPI analysis were performed on the identified differential proteins to determine the target protein.Results: Compared with the normal glomerular group,the MCD glomerular group had a total of 319 differentially expressed proteins(DEPs).Compared with the normal renal interstitial group,the MCD renal interstitial group has 4 DEPs.Compared with the normal tubule group,the MCD tubule group has 2 DEPs.Bioinformatics analysis of DEPs in the glomerulus group identified four proteins of interest: ACTN4,ZYX,CSK and CAN1.Significant differential expression of COPD,HPRT,FIBG and HEMO was found in the renal interstitium.Significant differential expression of GTR1 and WBP2 was found in the renal tubules.Conclusion: ACTN4,ZYX,CSK and CAN1 are potential biomarkers for early diagnosis of MCD.COPD,HPRT,FIBG,HEMO,GTR1 and WBP2 may be the key proteins interacting with the immune compartment of the MCD kidney.