| Objective To establish a passive Heymann nephritis(passive Heymann nephritis,PHN)rat model,application sinomenine intervention by observing the PHN rats proteinuria function,renal function,liver and kidney pathology,and explore mechanisms for deep development sinomenine provide experimental basis.Methods SD rats(male,body weight 130± 20mg)100 rats fed 1 week after adaptation,were randomly divided into normal control group and PHN rats production team.For modeling group rats 0.4ml/100g of body weight goat anti-rat serum Fx1A tail vein injection method The rat model of PHN.Normal control group injected intravenously equal volume of saline.Rat urine protein positive indicator for the formation of nephritis,a successful model rats were randomly divided into Sinomenine low dose group,middle dose group Sinomenine Sinomenine alkali and high dose groups,ramipril,model group.It began nine days after modeling,Sinomenine low,medium and high dose groups were daily press 20,40,60mg/kg body weight by intraperitoneal injection of sinomenine injection.Ramipril daily according to 5mg/kg body weight of ramipril oral suspension.Model group and the normal control group,etc.according to ramipril volume normal saline solution.Administration of the first 10 days,20 days,30 days at the end to collect urine,abdominal aortic blood after each group were sacrificed on day 10 and 20 respectively in the first five rats were sacrificed on day 30,measuring 24-hour urinary protein excretion,blood routine,blood biochemistry;stripping out the death renal tissue,light microscopy,immune pathology.Results 1.rat blood,urine change indicators:(1)administration of the first days 10,20,30,model group,treatment group,urinary protein excretion volume was higher(P<0.05);administration of section 10 day,sinomenine high-dose group compared with the model group(P<0.05);the first 20 days of administration,sinomenine,the high dose group were lower than model group(P<0.05);no statistical between treatment groups Learn the difference(P>0.05).Urinary protein excretion was a whole group Sinomenine<<trend model group,high dose group Sinomenine<<trend Sinomenine low dose group Sinomenine dose group ramipril.(2)serum albumin level:in each treatment group serum albumin levels gradually increased;serum albumin was Sinomenine group>ramipril>Trend model group;throughout the experiment sinomenine treatment group serum albumin sinomenine high dose group showed a>sinomenine dose group>trend sinomenine low dose group.(3)serum creatinine,white blood cells,aspartate aminotransferase,alanine aminotransferase:no significant difference(P>0.05)between the model group,the treatment group and the control group;no significant difference(P between the treatment group and model group>0.05);no significant difference(P>0.05)between the treatment groups.2.Renal pathological changes:clear(1)glomerular structure light microscope,model group,open capillary loop better,mesangial cells and mesangial matrix,no significant proliferation,glomerular basement membrane thickening with spikes formed basement membrane degeneration.Electron microscope examination revealed:30 days after modeling,model group rat kidney tissue visible more electron-dense material of varying sizes scattered in the glomerular basement membrane,epithelial cells under the deposition,uneven thickening of the basement membrane,forming spikes podocyte foot process fusion.(2)administration of the first 10 days,20 days,30 days,between the treatment group and model group,IgG deposition without kidney statistical difference between treatment groups(P>0.05).(3)Immunohistochemistry Podocin,Nephrin integral concept of density:each group tissue protein Podocin,Nephrin expression showed an increasing trend,Sinomenine group>ramipril>model group overall trend was.Conclusion Sinomenine can reduce urinary protein excretion PHN rats;Sinomenine not reduce the rat kidney PHN IgG deposition;sinomenine can increase rat Podocin PHN,expression Nephrin,repair,podocytes;sinomenine in treating PHN no significant side effects in rats during... |
PDF Full Text Request