| Objective To determine whether the anti-Ex160 antibody effectively suppresses the embryonation of the eggs in new zealand rabbits with Schistosomiasis japonica,which may provide an potential therapeutic strategy against liver fibrosis in diseases including schistosomiasis,and further study its mechanism.Methods New zealand rabbits were divided into 6 groups,PBS+Nc,PBS+Sj,PBS+Adjuvant+Sj,PBS+Buffer+Sj,Buffer+Adjuvant+Sj and Ex160+Adjuvant+Sj.Rabbit was immunized every two weeks for a total of 3 times,and the amount of antigen for each immunization is 400 ug per rabbit,collect blood at ear margins at 0w,2w,4w,6w,9w,11 w,and 14 w,respectively.We detected the levels of AST,ALT,HDL-C and anti-Ex160 antibody in serum of new zealand rabbits.Each rabbit was challenged with 100 ± 2 S.japonicum cercariae by abdominal skin penetration two weeks after the last vaccination.Eight weeks post challenge,all rabbits were sacrificed and the total adult worms recovered after perfusion of the portal vein.The egg burden and the maturity of miracidium in liver was determined.The assessment of liver fibrosis was calculated using sectioned liver tissue stained with sirius red staining.Results After immunization three times,We found the level of serum antibody immunized by rEx160 increased significantly by ELISA,which showed specific Ig G antibody against rEx160 was induced successfully.The liver fibrosis was significantly reduced in Ex160+Adjuvant+Sj group,indicating that rEx160 can effectively reduce hepatic fibrosis caused by schistosomiasis.However,there were no significant differences in the burdens of worm and egg,and eggs producted by each pair of worms in each group,and transaminase level at 0,3,5,and 8 weeks after schistosome infection did not change,suggesting that rEx160 did not affect the burdens of worm,egg and the damage of liver cells.We found a significant decrease in the maturity of mracidium in liver in the Ex160+Adjuvant+Sj group,and the serum HDL-C concentration in the Ex160+Adjuvant+Sj group was significantly higher than in the other infection control groups.Conclusion The data demonstrated immunization of rEx160 significantly decreased the liver fibrosis in schistosoma japonicum-infected rabbits.rEx160 can inhibit the eggs develop into mature miracidium.Objective To investigate the role of TIGIT signal in Th1/Th2 balance in the process of Schistosoma japonicum infection.Methods Male C57BL/6 mice were infected with cercaria,and healthy mice as the controls.The percentage of TIGIT+ cells,Ki67+CD3+CD4+TIGIT+cells,Ki67+CD3+CD4+TIGIT-cells,IFN-γ+CD3+CD4+ TIGIT+cells,IFN-γ+CD3+CD4+TIGIT-cells,IL-4+CD3+CD4+TIGIT+cells and IL-4+ CD3+CD4+TIGIT+cells of spleen in mice were evaluated by flow cytometry.Results The proportion of TIGIT+CD4+ T cells in the spleen from S.japonicum-infected(Sj)mice were higher than those in the normal uninfected(Nc)mice(Sj VS.Nc: 29.30%±0.70% VS.3.09%±0.50%,t=8.834,P=0.0002);However,no significant differences in the percentages of TIGIT+ CD8+ T cells between the Sj group(3.61%±0.26%)and the Nc group(3.58%±0.16%)were found(t=0.108,P=0.9192);in addition,no significant differences in the percentages of TIGIT+ non-T cells cells between the Sj group(1.86%±0.19%)and the Nc group(1.37%±0.17%)were found(t=1.931,P =0.1257).The further analysis showed that the proportion of Ki67 in the TIGIT+cells(17.03%±0.64%)was higher than that in the TIGITcells(6.59%±0.37%)(t=14.09,P =0.0001).The ratio of Th2/Th1 in the TIGIT+CD4+T cells(39.28%±3.75%)was higher than that in the TIGIT-CD4+ T cells(11.79%±1.64%)(t=6.721,P =0.0026).Conclusion Our data suggested that the TIGIT signaling may be involved in the development of Th2 responses in mice infected with S.japonicum. |
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