Long Noncoding RNA LINC01234 Functions As A Competing Endogenous RNA To Regulate CBFB Expression By Sponging MiR-204-5p In Gastric Cancer

Purpose: Long noncoding RNAs(lnc RNAs)have emerged as important regulators in a variety of human diseases,including cancers.However,the overall biological roles and clinical significance of most lnc RNAs in gastric carcinogenesis are not fully understood.We investigated the clinical significance,biological function and mechanism of LINC01234 in gastric cancer(GC).Experimental Design: Firstly,we analyzed LINC01234 alterations in gastric cancerous and noncancerous tissues through an analysis of sequencing data obtained from The Cancer Genome Atlas.Next,we evaluated the effect of LINC01234 on the GC cells proliferation and apoptosis,RNA-seq analysis of the downstream target genes regulated by LINC01234,and its regulation of mi R-204-5p by acting as a ce RNA.The animal model was used to support the in vitro experimental findings.Results: We found that LINC01234 expression was significantly upregulated in gastric cancer tissues and was associated with larger tumor size,advanced TNM stage,lymph node metastasis,and shorter survival time.Furthermore,knockdown of LINC01234 induced apoptosis and growth arrest in vitro and inhibited tumorigenesis in mouse xenografts.Mechanistic investigations indicated that LINC01234 functioned as a ce RNA for mi R-204-5p,thereby leading to the derepression of its endogenous target core-binding factor β(CBFB).Conclusions: LINC01234 is significantly over-expressed in GC,and LINC01234–mi R-204-5p–CBFB axis play critical role in GC tumorigenesis.Our findings may provide a potential new target for gastric cancer diagnosis and therapy.