The Clinical Study Of JianPi XiaoKe Recipe In Terms Of The Clinical Observation To T2DM And Experimental Study On Apoptosis Of Islet β-cells By MST1/PDX1 Pathway

Purpose:This article through clinical observation and experimental study to explore the efficacy of JianPiXiaoKeFang for type 2 diabetes mellitus(T2DM),the apoptosis detection of β-islet(MIN6).And the effect on expression of MTT1/PDX1.Materials and Methods1.Clinical study:80 patients with T2DM syndrome were divided into treatment group(40 cases)and control group(40 cases).The control group was treated with metformin hydrochloride tablets.The treatment group was treated with the Chinese medicine Jianpi Xiaoke Decoction on the basis of the same group as the control group.The treatment period was 60 days.Observed fasting blood glucose(FBG),postprandial 2h blood glucose(2hPG),glycosylated hemoglobin(HbA1C),blood lipids(TC,TG,HDL-C,LDL-C),body mass index(BMI),insulin levels,insulin resistance Changes in the index,beta cell function index,clinical symptoms of TCM and total curative effect of the disease.2.Experimental study:Islet β cells(MIN6)cells were divided into blank group,model group,Chinese medicine group and metformin group.The blank group was given low glucose medium(containing 5.5mmol/L glucose).The model group,Chinese medicine group and metformin group were given high glucose medium(containing 25mmol/L glucose)to simulate high sugar environment.The Chinese medicine group was given an aqueous solution of Jianpi Xiaoke Recipe,and the metformin group was given a metformin suspension for 48 hours.The optimal concentration of Jianpi Xiaoke Recipe,the survival rate of MIN6 cells,apoptosis and the expression of MST1,PDX1 mRNA and protein were screened.result:1.Clinical study:Of the 80 patients,77 were eventually included in the study,including 39 in the treatment group and 38 in the control group.After treatment,the syndrome-integrated group was lower than the control group(P<0.05),and the total effective rate of syndrome was higher than that of the control group(P<0.05).The total curative effect was better than the control group(P<0.05).The fasting blood glucose,postprandial 2h blood glucose,glycosylated hemoglobin treatment group was significantly lower than the control group(P<0.01),there was no significant difference between the fasting insulin treatment and the treatment before treatment(P>0.05),2h postprandial insulin treatment group compared with the control group The secretion increased(P<0.05),HOMA-β,HOMA-IR treatment group was significantly better than the control group(P<0.01),TC、TG、HDL-C、LDL-C treatment group was significantly lower than the control group(P<0.01).Blood routine,urine routine,stool routine,liver and kidney function,and electrocardiogram monitoring were performed in both groups,and no abnormalities were found.2.Experimental study:The experimental study found that the cell survival rate of the model group was significantly lower than that of the blank group(P<0.01),and the cell survival rate was the highest when the concentration of the traditional Chinese medicine was 0.001 mg/ml.The cell survival rate of the Chinese medicine group and the metformin group was significantly better than that of the model group(P<0.01).The total apoptosis rate of the Chinese medicine group and the metformin group was significantly lower than that of the model group(P<0.01).The expressions of MST1 and PDX1 mRNA in the Chinese medicine group and the metformin group were significantly better than those in the model group(P<0.01).The protein expressions of MST1 and PDX1 in the Chinese medicine group and metformin group were significantly better than those in the model group(P<0.01).in conclusion:In clinical studies,Jianpi Xiaoke Decoction combined with metformin can improve the total efficacy of T2DM disease,clinical symptoms,blood sugar,glycosylated hemoglobin,blood lipids and insulin levels,and improve insulin resistance,which is better than metformin alone.In the experimental study,Jianpi Xiaoke Recipe can promote islet β cell proliferation,inhibit apoptosis,decrease MST1 expression and increase PDX1 expression.Jianpi Xiaoke Decoction is effective and safe in the treatment of T2DM.It is speculated that its mechanism may be related to the inhibition of islet β apoptosis by interfering with MST1/PDX1 signaling pathway.