Effect Of MiR-486-5p Targeting TRPM2 On Autophagy And Apoptosis In Parkinson’s Disease Model Cells

ObjectiveObjective To investigate the effect of micro RNA-486-5p(miR-486-5p)on apoptosis and autophagy in Parkinson’s disease(PD)model induced by 1-methyl-4-phenylpyridinium ion(MPP~+).MethodsThe PD cell model was established by inducing SK-N-SH cells with MPP~+,and the expression of miR-486-5p and TRPM2 in PD cell model was detected by q RT-PCR and Western blot.miR-486-5p mimics,miR-NC,si-TRPM2,and si-NC were transfected into SK-N-SH cells.After transfection,the cells were divided into Con group,MPP~+group and MPP~++miR-486-5p group,MPP~++miR-NC group,MPP~++si-TRPM2 group,MPP~++si-NC group.The apoptosis rate of PD cell model was analyzed by flow cytometry.Western blot was used to detect the expression of autophagic protein markers LC3II,LC3I,mitochondrial function-related proteins Mfn2,NRF1 and apoptosis proteins Bcl-2,Bax and cleaved-caspase3.Dual luciferase reporter assays were used to validate the targeted regulation of miR-486-5p and TRPM2.ResultsT session on MPP~+-induced mitochondrial function-related proteins,autophagy-related protein expression,and apoptosis in SK-N-SH cells.ConclusionsOverexpression of miR-486-5p inhibited apoptosis and autophagy in The apoptosis rate of SK-N-SH cells treated with MPP~+was significantly increased(P<0.05),and the expression levels of LC3II,Bax and cleaved-caspase3 were significantly increased(P<0.05),while Mfn2,NRF1 and Bcl-2 proteins were observed.The expression level was significantly reduced(P<0.05).The expression level of miR-486-5p was decreased in PD cell model(P<0.05),and the expression level of TRPM2 was increased(P<0.05).Overexpression of miR-486-5p and interference of TRPM2 expression in PD cell model significantly decreased apoptosis rate and expression of LC3II,Bax,cleaved-caspase3 protein(P<0.05),and increased Mfn2,NRF1,Bcl-2protein expression(P<0.05).The dual luciferase reporter assay demonstrated that miR-486-5p can target the regulation of TRPM2expression.Overexpression of TRPM2 reversed the effect of miR-486-5p overexpr PD model cells by down-regulating the expression of TRPM2.