Isorhapontigenin Suppresses Interleukin-1β-Induced Inflammation And Cartilage Matrix Damage In Rat Chondrocytes

Objective: Osteoarthritis is a slowly progressive disease of joint pain and dyskinesia caused by senile multiple causes,and the onset of osteoarthritis is closely related to the inflammatory response and the destruction of the cartilage matrix.Isorhapontigenin(ISO)is a naturally compound drug in various plants,and it has proven its potential anti-inflammatory and antioxidant properties in some inflammatory diseases.However,the effect of ISO on osteoarthritis has not been proven,and its specific mechanism remains to be elucidated.This study will observe the effect of ISO on rat chondrocytes induced by IL-1β.Methods: 1.Chondrocytes were isolated,cultured and frozen,and rat chondrocytes were stimulated with IL-β(10ng / ml).2.Using CCK-8(cell counting Kit-8)method to measure the effects of ISO and IL-1β on the proliferation and toxicity of rat chondrocytes.3.Griess reaction and prostaglandin E2(PEG2)Elisa kit were used to measure the concentration of NO and PGE2.4.Reverse Transcription-Polymerase Chain Reaction(RT-PCR)was used to measure MMPs,adamts5,Collagen 2 and Aggrecan at gene expression in chondrocytes stimulated by IL-1β(10ng / ml)after the concentration gradient was added.5.Western blotting was used to determine the expression of i NOS,COX2,MMPs,ADAMTS5,Collagen 2,and Aggreecan at the protein level after ISO at a concentration gradient on rat chondrocytes stimulated by IL-1β(10ng / ml)Results: 1.The ISO at appropriate concentration has no toxicity and proliferation effect on cells.2.ISO can inhibit the increase of NO and PGE2 induced by IL-1β(10ng/ml)with concentration-dependent.3.ISO can inhibit the increase of MMPs and adamts5 stimulated by IL-1β(10ng/ml)at the gene level,and promote the upregulation of Collagen 2 and Aggreecan in a concentrationdependent manner.4.ISO down-regulates protein levels induced by IL-1β(10ng / ml),i NOS,COX2,MMPs,ADAMTS5 and promotes the expression of Collagen2 and Aggreecan in a concentrationdependent manner.5.ISO inhibits the expression of p-ERK and p-p38 in the MAPK pathway activated by IL-1 stimulation.Conclusion: ISO has anti-inflammatory properties of rat chondrocytes stimulated by IL-1 and maintains the chondrocyte phenotype.Objective: Osteoarthritis is a slowly progressive senile disease.Its prominent features are meniscus wear,narrowing of joint space,and osteophyte formation.We simulated the in vivo experiments in animals and used the cartilage mass of knee joints in SD rats Cultured in vitro,the changes of the cartilage surface with the addition of ISO to the cartilage mass stimulated by IL-1β were observed.Methods: 1.The cartilage mass of knee joint was removed from SD rats after cervical dislocation and sacrificed,and cultured in vitro.After being stimulated by IL-1β(10ng / ml),ISO was added to culture in basic medium.2.The in vitro cultured cartilage mass was subjected to HE staining.Results: The cartilage tissues of rats cultured in vitro were stained with HE,showing that the cartilage surface of the simple stimulation group was more rough than the ISO-stimulated group,and the cartilage surface was severely damaged.Conclusion: ISO has a protective effect on the cartilage surface damage of cartilage block cultured in vitro stimulated by IL-1β(10ng / ml).