The Mechanical Research That Chronic Oxidative Stress Promotes The Activity Of MCF-7 Breast Cancer Cells By Activating GSK3β/β-catenin Signal Pathway

Objective:To establish a chronic oxidative stress cell line model of MCF-7 breast cancer cells,observe the growth under this environment,and explore the effects of chronic stress status on breast cancer proliferation,migration and invasion of breast cancer and its possible mechanisms.Methods: The CCK-8 assay was used to detect the proliferation inhibition rate of MCF-7 under the conditions of acute oxidative stress and chronic oxidative stress.The ability of MCF-7 cells to migrate and invade under chronic oxidative stress conditions was examined by scratch assay and transwell assay.The level of IL-6 was determined by ELISA.Superoxide dismutase activity(SOD)and total antioxidant capacity(T-AOC)of MCF-7 under chronic oxidative stress model conditions were tested by kit.The expression levels of GSK3β/β-catenin signaling pathway-related proteins and RNA were determined by RT-PCR and Western Blot.Result: Compared with acute oxidative stress,under the condition of chronic oxidative stress,the value-adding ability of MCF-7 was enhanced,and the ability of SOD and T-AOC was further enhanced.Its ability to migrate and invade is stronger than that of wild-type and MCF-7 under acute oxidative stress.This result is consistent with IL-6 protein expression,GSK3β/β-catenin signaling pathway-associated protein and RNA expression.Conclusion: chronic oxidative stress can promote the proliferation and metastasis of MCF-7 breast cancer cells,and its mechanism may be related to the activation of GSK3β/β-catenin signaling pathway and the increase of IL-6 expression.